The Expression Analysis of miR-1 and miR-133 in Longissimus dorsi Muscle from Different Pig Breeds

XI Qian-yun1,ZHOU Lian-lian1,LI Hong-yi1,HAN Dong2,SHU Gang1,WANG Song-bo1, GAO Ping1,ZHU Xiao-tong1,WANG Xiu-qi1,JIANG Qing-yan1,ZHANG Yong-liang1*(1.College of Animal Science,South China Agricultural University,Guangzhou 510642,China; 2.College of Animal Husbandry and Veterinary,Changchun 130062,China)

stem-loop RT-PCR;meat quality traits;miR-1;miR-133;expression analysis

This study aimed to investigate the expression abundance of miR-1 and miR-133 in the highest longissimus dorsi muscle from 180-day-old pigs including Landrace,Lantang and Dahuabai pigs using Real-time quantification of microRNAs by stem-loop RT-PCR method.The results showed that miR-1 abundance was the highest in Lantang,and 4.7 times higher than that in Landrace and 6.4 times higher than that in Dahuabai pigs(P<0.05),respectively,but not significantly different(P>0.05) between Landrace and Dahuabai pigs.miR-133 abundance was also the highest in Lantang,followed by Landrace and Dahuabai pigs,the former two were significantly 7.2 and 5.7 times higher(P<0.05) than that in the latter,respectively,but there was no significant differences(P>0.05) between Landrace and Lantang pigs.The results indicate that miR-1 and miR-133 expression are specific among the pig breeds,suggesting that the abundance of miR-1 and miR-133 are associated with meat quality traits in pigs.