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当前位置: 首页 > 文章 > Leptin基因对陆川猪和大白猪产仔数的影响 畜牧兽医学报 2007,38 (6) 608-613
Position: Home > Articles > Effects of Leptin Gene on Litter Size in Luchuan and Large White Pig Chinese Journal of Animal and Veterinary Sciences 2007,38 (6) 608-613

Leptin基因对陆川猪和大白猪产仔数的影响

作  者：
张冰;杨公社;孙超;黄敏瑞
单  位：
西北农林科技大学动物科技学院;广西畜牧研究所
关键词：
leptin;陆川猪;大白猪;产仔数
摘  要：
根据leptin基因在GenBank中的已知序列设计两对引物,采用PCR-SSCP技术在陆川猪和大白猪群体中进行单核苷酸多态性(SNPs)检测,发现了两个SNPs位点,并对其不同基因型个体PCR回收产物进行测序,同时对这两个SNPs位点与产仔数进行了关联分析。结果表明:这两个SNPs位点分别是在第3 469碱基处发生了T→C突变和第3 714碱基处发生了T→G突变,这两个突变都是同义突变;T3714G SNP对陆川猪产仔数影响显著(P<0.05),而对大白猪产仔数影响不显著(P>0.05),T3 469C SNP对陆川猪和大白猪产仔数影响均不显著(P>0.05)。
译  名：
Effects of Leptin Gene on Litter Size in Luchuan and Large White Pig
作  者：
ZHANG Bing1,YANG Gong-she1,SUN Chao1,HUANG Min-rui2(1.College of Animal Science and Technology,Northwest A﹠F University,Yangling 712100,China;2.Guangxi Institute of Animal Science,Nanning 530001,China)
关键词：
leptin;Luchuan pig;Large White pig;litter sizes
摘  要：
Single nucleotide polymorphisms(SNPs) of the leptin gene were tested using PCR-SSCP in Luchuan and Large White pigs,and the effects of the leptin gene on litter size traits were also analyzed.Two pairs of primers for leptin gene were designed based on the known sequence in GenBank,and two SNPs were identified within the product amplified by PCR-SSCP and sequencing.The results showed that:(1) the polymorphisms were due to two synonymous single base mutation T to C and T to G at base position 3 469 and 3 714,respectively;(2)using least square analysis,it was seen that T3 714G SNP had significant effect on litter size in Luchuan pigs(P<0.05),but no effect in Large White pigs(P>0.05);T3469C SNP had no significant effect on litter size in Luchuan and Large White pigs(P>0.05).

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