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当前位置: 首页 > 文章 > 甘蓝短散布元件对转基因表达效果的研究 云南农业大学学报(自然科学) 2008,23 (5) 590-594
Position: Home > Articles > Effect of Short Interspersed Nuclear Element(SINE) of Cabbage on Transgene Expression In Transgenic Tobacco Journal of Yunnan Agricultural University(Natural Science) 2008,23 (5) 590-594

甘蓝短散布元件对转基因表达效果的研究

作  者：
杨正安;曹晖;王小佳;张应华
单  位：
云南农业大学园林园艺学院;西南大学园艺园林学院
关键词：
甘蓝;短散布元件(SINE);核基质结合区;转基因表达;烟草
摘  要：
为检验甘蓝短散布元件(SINE)对植物转基因表达的影响,采用PCR方法,从甘蓝基因组中克隆了一段短散布元件序列(Short Interspersed Nuclear Element,SINE),该SINE具有核基质结合区(matrix attachmentregion,MAR)的结构特征,将其构建到β-葡糖醛酸酶(β-glucuronidase,GUS)基因(uidA)的两侧翼,形成SINE调控的植物表达载体,采用农杆菌介导法,将含SINE序列和不含SINE序列的植物表达载体导入烟草中。对转基因植株进行GUS活性定量测定,结果表明,SINE表现出类似MAR的功能,可以提高外源uidA基因的表达水平,与不含SINE的转化植株相比,外源基因的平均表达水平提高了2倍,但转基因植株个体间表达水平存在较大的差异。
译  名：
Effect of Short Interspersed Nuclear Element(SINE) of Cabbage on Transgene Expression In Transgenic Tobacco
作  者：
YANG Zheng-an1,CAO Hui1,WANG Xiao-jia2,ZHANG Ying-hua1(1.Faculty of Landscape and Horticulture,Yunnan Agricultural University,Kunming 650201,China;2.Department of Horticulture and Landscape,Southwest University,Chongqing 400715,China)
关键词：
cabbage;short interspersed nuclear element(SINE);matrix attachment region(MAR);transgene expression;tobacco
摘  要：
In this study,a SINE fragment was cloned from cabbage(Brassica oleracea var.capitata L.) by PCR method in order to test its effect on transgene expression in plant.This SINE fragment has the structure and feature of matrix attachment region(MAR).The plant expression vector was constructed by SINE sequence used to flank the β-glucuronidase(GUS) report gene within the T-DNA of the binary vector.Vectors containing or lacking SINEs were then used to transform tobacco plants by Agrobacterium tumfaciens.The biologic activity of GUS was quantitatively determined;the result showed that the expression level of uidA gene could be improved by regulation of SINE fragment,and the function of SINE was similar to MAR.Furthermore,transgenic plants containing the SINE and flanking the GUS gene exhibited higher levels of transgene expression,compared with the transgenic plants that lacked the SINE.On average,plants transformed with SINE-containing vector expressed GUS at level 2-fold higher than the controls.However,the expression differences among individual transformants were still obvious.

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