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当前位置: 首页 > 文章 > 西马特罗人工抗原的合成及鼠源多克隆抗血清的制备 华北农学报 2010,25 (4) 101-105
Position: Home > Articles > Synthesis of Cimaterol Artificial Antigen and Development of Its Polyclonal Antiserum Acta Agriculturae Boreali-Sinica 2010,25 (4) 101-105

西马特罗人工抗原的合成及鼠源多克隆抗血清的制备

作  者：
职爱民;刘庆堂;李青梅;杨苏珍;胡骁飞;柴书军;邓瑞广;张改平
单  位：
河南省农业科学院农业部动物免疫学重点实验室河南省动物免疫学重点实验室
关键词：
西马特罗;人工抗原;合成;多抗血情
摘  要：
拟合成并鉴定西马特罗(Cimaterol,CIM)人工抗原,通过动物免疫生产亲和力高、特异性好的鼠源CIM多克隆抗血清。通过重氮化方法将西马特罗偶联于载体蛋白BSA和OVA上,分别合成免疫抗原BSA-CIM和包被抗原OVA-CIM,并采用紫外分光光度法和SDS-PAGE进行了鉴定。结果表明,半抗原CIM和载体蛋白偶联成功。动物免疫试验结果显示,6只小鼠效价均达1×10-3以上,且4号小鼠多抗血清抗CIM的IC50为86.9ng/mL,表明成功获得了高效价、特异性好、亲和力高的的鼠源抗CIM多抗血清。
译  名：
Synthesis of Cimaterol Artificial Antigen and Development of Its Polyclonal Antiserum
作  者：
ZHI Ai-min,LIU Qing-tang,LI Qing-mei,YANG Su-zhen,HU Xiao-fei, CHAI Shu-jun,DENG Rui-guang,ZHANG Gai-ping (Key Laboratory of Animal Immunology of the Ministry of Agriculture,Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)
关键词：
Cimaterol; Artificial antigen; Synthesis; Polyclonal antiserum
摘  要：
The goal of this study was to synthesize artificial antigen of cimaterol and obtain its mice polyclonal antiserum. Artificial antigen BSA-CIM and OVA-CIM were synthesized using diazotization by linking carrier proteins BSA and OVA to CIM. The antigen BSA-CIM and OVA-CIM was identified by ultraviolet scanning,SDS-PAGE. The results showed that the hapten CIM was successfully linked to carrier proteins. The animal immunization results showed that six BALB /c mice indirect ELISA titer against CIM were above 1 × 10 -3 and the IC50 of No. 4 mice was 86. 9 ng /mL and the high-titer,sensitivity CIM polyclonal antiserum has been generated. The artificial antigen of CIM and its mice polyclonal antiserum laid a foundation base for product the monoclonal antibodies and rapid test reagent against CIM.

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