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Position: Home > Articles > Applying Matrix to Quantify Cross-contamination of Aeromonas spp.among Different Medium Transactions of the Chinese Society for Agricultural Machinery 2015,46 (4) 219-225+272

不同介质间采用矩阵量化气单胞菌的交叉污染

作  者:
董庆利;王海梅;Pradeep K Malakar;刘阳泰
单  位:
上海理工大学医疗器械与食品学院;食品研究所肠道健康与食品安全组
关键词:
气单胞菌;交叉污染;矩阵;介质
摘  要:
为定量描述气单胞菌在不同介质间的转移及变化过程,基于单个细菌在每次转移过程中只能被转移到一个介质表面的假设,采用矩阵方式将气单胞菌的转移路径及被转移的可能性集中于一个二维的数据表格之中,并对不同场景下气单胞菌在不同介质表面的交叉污染水平进行分析。结果表明,采用矩阵方式不仅可用于探究交叉污染对细菌受体表面带菌量的变化,同时可定量分析细菌供体表面带菌量的变化,如在分割冷却猪肉前、后,猪肉中气单胞菌的带菌量平均值分别为3.02 lg CFU/g、1.82 lg CFU/g。对不同场景下各介质带菌量的量化结果证实:分割冷却猪肉后,若及时对被污染的案板、刀具等介质进行清洗或更换,可有效降低交叉污染水平并可预防二次交叉污染的发生。
译  名:
Applying Matrix to Quantify Cross-contamination of Aeromonas spp.among Different Medium
作  者:
Dong Qingli;Wang Haimei;Pradeep K Malakar;Liu Yangtai;School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology;Gut Health and Food Safety,Institute of Food Research;
关键词:
Aeromonas spp.Cross-contamination Matrix Medium
摘  要:
The transfer approach and transfer probability of Aeromonas spp.under various food-handling scenarios were centralized in a two-dimensional data table by applying matrix method,which aimed at quantifying cross-contamination of Aeromonas spp.among different food-contact surfaces.Results showed that the number of Aeromonas spp.on donor and recipient surface could be calculated simultaneously,for instance,the average values of Aeromonas spp.in chilled pork before and after cross-contamination were3.02 lg CFU/g and 1.82 lg CFU/g,respectively.Results also confirmed that proper conducts after splitting pork including cleaning contaminated boards,knives,hands and/or just changing them into clean ones before splitting Brassica chinensis could effectively reduce cross-contamination level.The study of microbial cross-contamination could be helpful for setting good hygienic practice guidelines for consumers,and it could provide a theoretical basis for assessing the potential risk of cross-contamination.

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