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Position: Home > Articles > Genetic diversity based on microsatellite markers in five channel catfish(Ictalurus punctatus) populations Journal of Aquaculture 2011,32 (1) 24-30

五个斑点叉尾群体的微卫星遗传多样性分析

作  者:
赵沐子;李茜;秦钦;蔡永祥;许志强;王明华;陈校辉;葛家春;边文冀
单  位:
苏州大学水产研究所;江苏省淡水水产研究所
关键词:
斑点叉尾;微卫星;遗传育种;遗传多样性
摘  要:
用8对微卫星引物对1997-2004年引进的5个斑点叉尾群体进行遗传多样性分析,计算并统计等位基因数、多态信息含量(PIC)、杂合度、遗传相似性系数、遗传距离等参数。实验显示8个微卫星位点在5个斑点叉尾群体中共检测到42个等位基因,平均期望杂合度为0.6338~0.7320,表明其遗传多样性程度处于中等偏上水平。平均多态信息含量为0.5756~0.6869,说明基因座为高度多态基因座(PIC>0.5)。群体间遗传相似系数为0.7504~0.9203。聚类分析显示,04群体与其他4个群体的亲缘关系较远。结果表明:引进的5个斑点叉尾群体均具有较高的遗传多样性,遗传信息丰富,遗传变异大,为良好的育种材料。
译  名:
Genetic diversity based on microsatellite markers in five channel catfish(Ictalurus punctatus) populations
作  者:
Zhao muzi1,Li qian2,Qin qin1,Cai yongxiang1,Xu zhiqiang1,Wang minghua1,Chen xiaohui1,Ge jiachun1,Bian wenji1(1.Freshwater Fisheries Research Institute of Jiangsu Province,Nanjing 210017,China;2.Fisheries Research Institute of Soochow University,Suzhou 215123,China)
关键词:
channel catfish;microsatellite markers;genetic breeding;genetics diversities
摘  要:
To provide background on the genetics of cultured channel catfish(Ictalurus punctatus)and basic information for breeding,we surveyed five channel catfish populations using 8 microsatellite loci to estimate the genetic variation.A total of 42 alleles were detected.Mean expected heterozygosity(He)ranged between 0.6338 and 0.7320,and mean polymorphism information content(PIC)ranged between 0.5756 and 0.6869,which were high polymorphism(PIC>0.5),the genetic similarity coefficient was from 0.7504 to 0.9203,the cluster analysis by UPGMA using the MEGA software package(version 4.0)indicated high similarity among all populations except population 04,indicating that these five channel catfish populations were highly polymorphic,abundant in genetics information and had a great potential of selective breeding.

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