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Position: Home > Articles > 一种水稻穗腐病新病原的鉴定 Journal of Henan Agricultural Sciences 2019 (1) 93-98

一种水稻穗腐病新病原的鉴定

作  者：
陈利军;王春生;田雪亮;智亚楠;刘焱琨;卫云飞
单  位：
信阳农林学院农学院;信阳农林学院;信阳农林学院 农学院;河南科技学院资源与环境学院
关键词：
水稻穗腐病;新病原;草酸青霉;病原菌鉴定
摘  要：
水稻穗腐病在我国的发生危害逐年加重,在对河南省信阳市水稻穗腐病的调查中,发现部分发生穗腐病水稻谷粒外颖开裂,外露籽粒伴有蓝绿色霉层,有别于其他病原菌引起的穗腐病症状,为了解引起该病害的病原真菌及其特性,对其进行了研究.通过病样标本室内分离,获得分离物,并对其进行致病性测定、形态学鉴定和多基因系统发育分析.结果表明,分离得到的菌株R1形态学特征与草酸青霉(Penicillium oxalicum)一致,在结合ITS、BenA和CaM序列构建的系统发育树中,菌株R1和草酸青霉处于同一分支,支持率为100%,故将此菌株鉴定为草酸青霉.致病性测定表明,草酸青霉为水稻穗腐病新病原菌.
作  者：
CHEN Lijun;WANG Chunsheng;TIAN Xueliang;ZHI Ya'nan;LIU Yankun;WEI Yunfei;Agriculture College, Xinyang Agriculture and Forestry University;Henan Provincial South Henan Crop Pest Green Prevention and Control Academician Workstation;College of Resources and Environment, Henan Institute of Science and Technology;
关键词：
Rice spikelet rot disease;;New pathogen;;Penicillium oxalicum;;Pathogen identification
摘  要：
In China,the damage caused by rice spikelet rot disease(RSRD) was increased by years.It was found that exposed grain of some rice plants with dehiscent glume had blue-green mold,which was different from RSRD symptoms caused by other pathogens during the investigation of that in Xinyang City of Henan Province.The isolate from collected samples in laboratory was tested for pathogenicity and identified by morphological characteristics and multigene phylogenetic analysis.The results showed that the isolate R1 was identified as Penicillium oxalicum based on the same morphological characteristics and identical branch with 100% support rate in phylogenetic tree constructed with sequences of ITS,BenA and CaM.The test for pathogenicity indicated that Penicillium oxalicum was a new pathogen of RSRD.

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