关键词：
‘西伯利亚’百合;LsAOS;基因克隆;表达分析
摘 要：
丙二烯氧化合酶(allene oxide synthase,AOS)基因是茉莉酸(JA)合成途径中第一个特异性酶,具有调控植物体内JA合成积累的重要作用。本研究通过RACE技术克隆得到了‘西伯利亚’百合Lilium‘Siberia’中的AOS基因,并将其命名为LsAOS。该基因全长1 542 bp,编码513个氨基酸,其蛋白序列与小果野芭蕉的同源性最高,属于不稳定亲水蛋白。通过对‘西伯利亚’百合4个不同花期,9个不同组织器官进行实时荧光定量PCR(q-PCR)后发现,LsAOS在花蕾期中的表达量最高,随着花朵的逐渐开放表达量逐渐下降;LsAOS在叶片中表达水平最高,其次是内瓣、根、茎等。JA参与植物生长发育的全过程,与植物抗逆性和次生代谢反应有着密不可分的联系。AOS基因是JA途径中的关键基因之一,对JA的合成有着重要的调控作用。
译 名：
Cloning and Expression Analysis of Allene Oxide Synthase LsAOS Gene in Lilium ‘Siberia’
作 者：
Wu Qi;Leng Pingsheng;Hu Zenghui;College of Landscape Architecture, Beijing University of Agriculture;Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit Trees;
单 位：
Wu Qi%Leng Pingsheng%Hu Zenghui%College of Landscape Architecture, Beijing University of Agriculture%Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit Trees
关键词：
Lilium ‘Siberia’;;LsAOS;;Gene cloning;;Expression analysis
摘 要：
Allene oxide synthase(AOS) gene is a specific enzyme in the synthesis pathway of jasmine acid(JA),which has the important role to regulate the accumulation of JA synthesis in plants. In this study, AOS gene in Lilium‘Siberia'was cloned by rapid amplification of c DNA ends(RACE) technology and it was named as Ls AOS.The gene was 1 542 bp and encoded 513 amino acids. Its protein sequence had the highest homology with Musa acuminata, belonging to unstable hydrophilic protein. Through the real-time fluorescent quantitative PCR(q-PCR)experiment, we measured the Ls AOS gene expression quantity in four different flowering phases and nine organs.The results showed that Ls AOS gene expressed the highest in the budding phase, and the expression quantity decreased gradually with the opening of flowers; Ls AOS gene had the highest expression level in the leaves,followed by inner petals, roots, stems and so on. JA participated in the whole process of plant development, which was closely related to plant resistance reaction and secondary metabolism. AOS gene was one of the key genes in the JA pathway and played an important role in regulating the synthesis of JA.
