Position: Home > Articles > Mutation breeding of pleuromutilin irradiated by heavy ion beam
Journal of Traditional Chinese Veterinary Medicine
2015
(6)
29-30
重离子辐照截短侧耳素产生菌的诱变选育
作 者：
王学红;郝宝成;赵晓斌;刘宇;梁剑平
单 位：
中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室
关键词：
截短侧耳素产生菌;重离子;辐照;筛选
摘 要：
利用重离子辐照对截短侧耳素产生菌进行诱变,筛选高产菌株,采用~(12)C~(6+)重离子束,剂量分别为20 Gy、30 Gy、40 Gy、50 Gy。结果显示重离子辐照的最适剂量为40 Gy。筛选出一株截短侧耳素高产菌株K40-3,摇瓶效价达到16 120μg/mL,较出发菌株提高25.3%。
译 名：
Mutation breeding of pleuromutilin irradiated by heavy ion beam
作 者：
WANG Xue-hong;HAO Bao-cheng;ZHAO xiao-bin;LIU Yu;LIANG Jian-ping;Key Laboratory of New Animal Drug Project,Gansu Province/Key Laboratory of Veterinary Pharmaceutics Discovery,Ministry of Agriculture/ Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences;
关键词：
pleuromutilin producing bacteria;;heavy ion;;irradiation;;screening
摘 要：
The pleuromutilin producing bacteria were induced by heavy ion irradiation to screen high-yield strains.~(12)C~(6+)heavy ion beam was used for twice irradiations at the dosage of 20 Gy,30 Gy,40 Gy and 50 Gy,respectively.The results showed that the optimal dosage was 40 Gy.A strain of pleuromutilin producing strain of K40-3 was selected out with a titer in shake flask of 16 120 μg/mL,which was 25.3%higher than that of parent strain.
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