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Position: Home > Articles > Mutagenesis and Breeding of Fermentation Strains about Cold Water Infused Tea FOOD SCIENCE 2008,29 (2) 244-246

冷泡茶发酵菌种的诱变育种

作  者:
杨坚;孙永康;潘科
单  位:
西南大学食品科学学院
关键词:
纤维素酶;诱变;紫外线;亚硝酸钠
摘  要:
对从茶叶中筛选出的1株纤维分解菌SW3进行紫外线和亚硝酸钠诱变,用刚果红透明圈平板法来进行初筛,再采用摇瓶复筛,从中筛选出发酵液滤纸酶活力高的突变株SW355。突变株SW355的发酵液滤纸酶活力比出发菌株SW3提高了62.9%,且其遗传性状稳定。
译  名:
Mutagenesis and Breeding of Fermentation Strains about Cold Water Infused Tea
作  者:
YANG Jian,SUN Yong-kang,PAN Ke (College of Food Science, Southwest University, Chongqing 400716, China)
关键词:
cellulase;mutation;UV;NaNO2
摘  要:
A cellulase-decomposing bacterium SW3 was screened out from tea, and mutagenized with UV and NaNO2. The first isolation was conducted according to the method of distinct zone of clearing in cellulase-congo red agar medium. The second isolation was conducted according to the test of filter paper enzyme activity through liquid fermentation. SW355 is 62.9% higher than the original strain SW3, and it has a stable property in transmissibility.

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