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当前位置: 首页 > 文章 > 苹果采后青霉病拮抗菌株的分离、鉴定和低能N~+注入诱变 北方园艺 2015 (21) 116-120
Position: Home > Articles > Isolation and Identification of Antagonistic Bacteria Against Postharvest Blue Mold Decay of Apple and Its Mutation by Low Energy N~+ Implantation Northern Horticulture 2015 (21) 116-120

苹果采后青霉病拮抗菌株的分离、鉴定和低能N~+注入诱变

作  者：
付瑞敏;邢文会;谷亚楠;薛婷婷;郭彦钊;陈五岭
单  位：
河南教育学院生命科学学院;西北大学生命科学学院
关键词：
苹果;采摘后;青霉病;解淀粉芽胞杆菌;低能N+注入
摘  要：
以苹果表面分离到拮抗扩展青霉的菌株BA-16为试材,经形态学、生理生化及16S rRNA序列分析,该菌被鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。为了增强该菌株防治青霉病的能力,通过低能N+注入技术对其进行诱变选育,获得抑菌性能显著提高且遗传性能稳定的突变株BA-16-8。为检测该突变株防治青霉病的增强效果,分别对野生株BA-16和突变株BA-16-8的菌悬液和无细胞发酵液进行果实生防检测。结果表明:突变株的菌体及代谢产物的抗病性能显著高于野生菌株,对于苹果采后青霉病的生物防治具有良好的应用开发前景。
译  名：
Isolation and Identification of Antagonistic Bacteria Against Postharvest Blue Mold Decay of Apple and Its Mutation by Low Energy N~+ Implantation
作  者：
FU Ruimin;XING Wenhui;GU Yanan;XUE Tingting;GUO Yanzhao;CHEN Wuling;Department of Life Science,Henan Institute of Education;College of Life Science,Northwest University;
关键词：
apple;;postharvest;;blue mold decay;;Bacillus amyloliquefaciens;;low energy N+implantation
摘  要：
Taking an antagonistic strain BA-16 which could inhibit Penicillium expansumisolated from apple surface as metarial,phenotypic,physiological,biochemical and phylogenetic(16SrRNA)charactors were studied.The strain BA-16 was identified as Bacillus amyloliquefaciens.In order to enhance its antagonistic capability,the mutation of BA-16 was carried out by using low energy N+implantation.After the mutation,the mutant BA-16-8,which showed the strongest antagonist capability and stable hereditary stability was selected out.Aiming at estimating the increasing biological control effects of apple blue mold decay by mutant BA-16-8,the inhibition effects of bacterial broth and cell-free fermentation broth of mutant and wild-type strain were studied by biological control trial on fruit.The results showed that comparing with its wild type,the mutant displayed significantly higher effect on controlling apple blue mold disease.Therefore,the mutant BA-16-8will be a potential biological control agent against apple blue mold decay.

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