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当前位置: 首页 > 文章 > 耐超高压胁迫副溶血性弧菌的逆境耐受性 食品科学 2014,35 (7) 164-169
Position: Home > Articles > Susceptibility of Pressure-Resistant Vibrio parahaemolyticus to Various Environmental Stresses FOOD SCIENCE 2014,35 (7) 164-169

耐超高压胁迫副溶血性弧菌的逆境耐受性

作  者：
黄小鸣;童钰;马君妍;祝凯丽;周杨武;陆海霞;励建荣
单  位：
浙江工商大学食品与生物工程学院;渤海大学化学化工与食品安全学院
关键词：
副溶血性弧菌;耐压菌株;逆境
摘  要：
目的:探讨水产品中耐超高压胁迫的副溶血性弧菌对高压及其他逆境环境的耐受性。方法:以80~250 MPa超高压处理原始敏感菌株(Vibrio.parahaemolyticus ZJGSMC001),筛选得到耐高压菌株(V.parahaemolyticus ZJGSPR001),以其他逆境处理,分析二者对高压和其他逆境的耐受性差异。结果:以250 MPa压力胁迫处理,耐压菌株存活量比原始菌株高2(lg(CFU/mL))。3℃以下原始菌株生长速率为负值,45℃以上原始菌株不能存活,耐压菌株则生长良好,1℃和48℃时仍可存活。在NaCl质量浓度高达11.5 g/100 mL时,原始菌株为负生长,耐压菌株仍能生长。耐压菌株对有机溶剂和有机酸的耐受性增强,其中乙醇体积分数12%、丙酮体积分数9%、甲苯体积分数0.75%、柠檬酸3 mg/mL和乳酸体积分数1.5%时,原始菌株全部致死,耐压菌株仍存活。结论:原始菌株对压力较敏感,而耐压菌株经超高压胁迫处理后,除对高压的耐受能力提高外,对其他逆境(如温度、氯化钠、有机溶剂、有机酸)的耐受性也有所增强。
译  名：
Susceptibility of Pressure-Resistant Vibrio parahaemolyticus to Various Environmental Stresses
作  者：
HUANG Xiao-ming;TONG Yu;MA Jun-yan;ZHU Kai-li;ZHOU Yang-wu;LU Hai-xia;LI Jian-rong;Food Safety Key Laboratory of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University;Food Safety Key Laboratory of Liaoning Province, College of Chemistry, Chemical Engineering and Food Safety, Bohai University;
关键词：
Vibrio parahaemolyticus;;pressure-resistant bacteria;;environmental stresses
摘  要：
Objective: The objective of this study was to observe the susceptibility of Vibrio parahaemolyticus to various environment stresses as influenced by high hydrostatic pressure treatments. Methods: A pressure-sensitive strain ofV. parahaemolyticus(ZJGSMC001) isolated from seafoods was repeatedly treated with high hydrostatic pressures ranging from 80 to 250 MPa, and the surviving cells were selected as pressure-resistant strains(ZJGSPR001). The tolerance of V. parahaemolyticus to subsequent adverse conditions was investigated in the pressure-sensitive strains as well as the pressureresistant strains. Results: When exposed to a pressure of 250 MPa, the survival of pressure-resistant strains was 2(lg(CFU/mL)) higher than that of pressure-sensitive strains. When exposed to low temperature(1 ℃) and high temperature(45 ℃), pressuresensitive strains could not survive, while the pressure-resistant ones grew well and still survive at 1 ℃or 48 ℃. Pressuresensitive strains could not grow at high salt concentration such as 11.5 g/100 mL NaCl. However, pressure-resistant bacteria could survive and grow well. Pressure-resistant strains were more resistant to organic solvent and organic acids compared to the pressure-sensitive ones. When exposed to alcohol(12%), acetone(9%), toluene(0.75%), citric acid(3 mg/mL) and lactic acid(1.5%), the pressure-sensitive bacteria were totally dead, while the pressure-resistant cells remained alive. Conclusion: The pressure-resistant V. parahaemolyticus is more tolerant to environment stresses such as temperature, NaCl solution, organic solvent and organic acids than the pressure-sensitive strains.

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