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Position: Home > Articles > Identification of causal agent of bacterial boll rot in cotton from Xinjiang province Acta Phytopathologica Sinica 2008,38 (3) 238-243

新疆棉花细菌性烂铃病病原菌鉴定

作  者:
刘雅琴;任毓忠;李国英;丁胜利;吉丽丽;张恒
单  位:
石河子大学农学院植物保护系兵团绿洲生态农业重点实验室
关键词:
棉花烂铃;细菌性病害;病原鉴定;成团泛生菌
摘  要:
2006~2007年在棉花结铃盛期,从新疆奎屯和石河子等地的不同棉田中采集细菌性烂铃病样,分别从病部棉绒、种子和铃壳上分离纯化得到了20个细菌菌株;使用针刺法接种棉铃后,所有供试菌株均引起与田间自然发病相一致的症状;从中选取8个代表性菌株进行了形态特征、培养特性、生理生化反应的观察和测定,同时用细菌16S rDNA通用引物进行了PCR扩增和序列测定,登录GenBank比对后,将其鉴定为成团泛生菌(Pantoea agglomerans)。该病原菌在棉铃上的危害在国内尚属首次报道。
译  名:
Identification of causal agent of bacterial boll rot in cotton from Xinjiang province
作  者:
LIU Ya-qin,REN Yu-zhong,LI Guo-ying,DING Sheng-li,JI Li-li,ZHANG Heng(Department of Plant Protection,Agricultural College of Shihezi University and Key Oasis Eco-Agriculture Laboratory of Xinjiang,Shihezi University,Shihezi 832003,China)
关键词:
boll rot of cotton;bacterial disease;pathogen identification;Pantoea agglomerans
摘  要:
Bacteria were isolated from diseased velveteen,cotton seed and legumen of cotton bolls,collected in a field in Kuitun and Shihezi of Xinjiang from 2006 to 2007 and tested for the ability to cause comparable boll rot symptoms in cotton fruit growth in a greenhouse.Some disease symptoms were reproduced with isolated bacteria suspension of 108 cfu/mL when cotton were inoculated one week.Pathogenic isolates were identified as Pantoea agglomerans based on later morphological characterizations,physiological and biochemical reactions and 16S ribosomal DNA sequence analysis(100% nucleotide identity).This is the first report that P.agglomerans is the pathogen of cotton roll rot in China.

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