中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 具有解钾活性假单胞菌的分离与鉴定 安徽农业科学 2016,44 (24) 17-19
Position: Home > Articles > Isolation and Identification of a Potassium-releasing Strain of Pseudomonas sp. Journal of Anhui Agricultural Sciences 2016,44 (24) 17-19

具有解钾活性假单胞菌的分离与鉴定

作  者：
罗雯;石路怀
单  位：
南昌师范学院生物系;西安文理学院生物与环境工程学院
关键词：
解钾菌;分离;鉴定;假单胞菌
摘  要：
[目的]分离并鉴定具有解钾活性假单胞菌。[方法]以农田土壤为样本,在钾长石粉为唯一钾源的选择性培养基上分离并纯化解钾菌,并通过形态观察和16S r DNA序列分析对分离到的细菌进行鉴定。[结果]经梯度稀释涂布和平板划线分离,经初筛获得8株生长良好并具有解钾透明圈的细菌。将初筛后获得的细菌菌株进行发酵培养,利用原子吸收分光光度法测定发酵上清液中的速效钾含量,从中筛选出解钾能力较强的1株假单胞菌K3。通过形态观察发现,该菌株为革兰氏阴性杆菌。16S r DNA序列分析结果表明,该菌株与荧光假单胞菌F113亲缘关系最近,初步确定该菌株属假单胞菌属。[结论]该研究为微生物钾肥的开发提供了新的试材。
译  名：
Isolation and Identification of a Potassium-releasing Strain of Pseudomonas sp.
作  者：
LUO Wen;SHI Lu-huai;Biology Department,Nanchang Normal University;School of Biological and Environmental Engineering,Xi'an University of Arts and Science;
关键词：
Potassium bacteria;;Isolation;;Identification;;Pseudomonas sp.
摘  要：
[Objective]To isolate and identify a potassium-releasing strain of Pseudomonas sp..[Method]With farmland soil as the sample,potassium-releasing bacteria was isolated and purified on the selective medium in which potash feldspar powder was used as the only source of potassium. Morphologic observation and 16 S r DNA gene sequence analysis were used to identify the isolated strains.[Result]By using the methods of gradient dilution coating and streaking inoculation,eight presumed potassium-releasing bacteria strains exhibiting clear zones were selected and inoculated into fermentation medium. The potassium contents in supernatant were determined with flame atomic absorption spectrophotometry method. Among them,a strain named K3 was verified to be with relatively high potassium-releasing ability cause the potassium contents in the supernatant of fermentation culture greatly increased comparing to the control sample. Through morphological observation it was verified to be Gramnegative rod bacteria. The 16 S r DNA gene sequence analysis result showed that it had most close genetic relationship with Pseudomonas fluorescens F113,thus tentatively identified as Pseudomonas sp.. [Conclusion] This research provides new test materials for the development of microbial potash fertilizer.

相似文章

导出引用
计量
文章访问数: 19
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46