Eexpressions and significance of the Caspase- 3 and Caspase- 9 in the transplanted tumor model of the PC- 12 cells

LI Xiao-ying;YANG Ci-qing;WANG Cong-rui;FU Su-lei;LIN Jun-tang;College of Life Science and Technology,Xinxiang Medical University;Key Laboratory of Medical Regeneration Tissue of Henan Province,Xinxiang Medical University;

PC-12 cells;;cell transplantation;;tumor;;Caspase-3;;Caspase-9

To detect the expressions of the Casepase- 3 and Caspase- 9 in the tumor tissue at different time points base on the establishment of tumor transplantation model of PC- 12 cells,and explore the role and significance of the Casepase- 3 and Caspase- 9 in the process of tumor development. The PC- 12 cells with a density of 1 × 107/ m L in the exponential phase were inoculated into the axillary subcutaneous region of the forelimb of Kunming white mouse on the seventh day of birth. The tumor mass and adjacent tissue were removed together at different time points including 7,14 and 21 days after inoculation,respectively; and then were fixed with paraformaldehyde( PFA) and embedded by embedding medium to be preformed the frozen sections. A fluorescent immunohistochemical method was used to detect the expressions of Caspase- 3and Caspase- 9. The results showed that in the Kunming white mouse on the seventh day of birth,the formation of tumor mass was obviously observed at 7 days after inoculating the PC- 12 cells and the tumor mass was the biggest at 14 days after inoculation,but decreased at 21 days,and the tumor mass almost disappeared at 28 days. Furthermore,the Caspase- 3 and Caspase- 9 showed low expressions in the control muscle group. The Caspase- 3 and Caspase- 9 showed positive expressions in the solid tumor tissues at 7 days after transplanting the tumor,and then the expressions were reduced while strengthened in the adjacent tissues at 14 days,but the expressions in the adjacent tissue were also reduced at 21 days. The results indicate that the real tumor transplantation model can't be constructed in the ordinary Kunming white mouse,while the tumor mass can also be generated in a short time. In addition,the expressions of the Caspase- 3 and Caspase- 9 have obvious differences in the region and amount at different time points,but the variations in the expression position and amount have similarity in the Caspase- 3 and Caspase- 9.