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Isolation and Characterization of a New Diterpenoid Compound from Agastache rugosa

作者：

Guoxin Tian;Liu Cun-fang;Lai Pu-hui;Sun ZhiFeng;Zhao Hu

单位：

Environmental Sciences,Shaanxi University of Technology,Hanzhong,Shaanxi 723000,China;Environmental Sciences,Shaanxi University of Technology,Hanzhong,Shaanxi 723000,Chin

关键词：

terpenoids;extracted;nm;rugosa;longikaurin;leave

摘要：

[Objective] To extract and isolate terpenoids from the leaves and branches of wild Agastache rugosa in Qinba mountainous area,and to study on the structure of those terpenoids.[Method] The powders of A.rugosa leaves and branches were extracted with 95% ethanol,the concrete was dispersed in water and then extracted by petroleum ether,ethyl acetate,butanol in turn,the terpenoids in the ethyl acetate extracts were isolated with silica gel column chromatography,and then characterized by TLC,IR,UV,NMR and x-ray diffraction.Its crystal structure was also determined by single crystal x-ray diffraction.[Result] This diterpenoid was Longikaurin D,the crystal of Longikaurin D belonged to orthorhombic crystallographic system,space group P2(1)2(1)2(1),and cell parameters:a=0.845 1(4) nm,b=1.061 5(5) nm,c=2.237 1(10) nm,α = β = γ = 90.00°,Mr=404.43,V=2.007(16) nm3,Dc=1.352 mg/m3,Z=4,F(000)=872,μ=0.103/mm,R1=0.076 7,ωR2=0.146 8.[Conclusion] Longikaurin D was extracted from A.rugosa for the first time,which provided scientific basis for developing and utilizing the terpenoids in A.rugosa.

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Isolation and Characterization of a New Diterpenoid Compound from Agastache rugosa

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