Position: Home > Articles > Establishment of a High-Throughput Fluorescence Multiplex PCR Method for Detection of Genetically Modified Rice Events
FOOD SCIENCE
2016,37
(20)
187-190
复合PCR荧光检测方法检测转基因水稻品系
作 者：
张明哲;张晓峰;徐莉莉;陈笑梅;周圆;尹文秀;陈吴健;李孝军
单 位：
浙江省检验检疫科学技术研究院;舟山出入境检验检疫局
关键词：
复合聚合酶链式反应;毛细管电泳;转基因水稻;品系鉴定
摘 要：
建立一种高通量的转基因复合聚合酶链式反应(polymerase chain reaction,PCR)荧光检测方法,该方法通过对转基因检测内源、外源及品系基因检测引物进行荧光标记,经复合PCR扩增和毛细管电泳荧光检测,实现特异性强、高通量检测。结果表明,复合PCR荧光检测方法检测4种转基因水稻品系特异性好,并且灵敏度达到0.1%,可以在进出口检验检疫、食品安全监测等领域进行应用。
译 名：
Establishment of a High-Throughput Fluorescence Multiplex PCR Method for Detection of Genetically Modified Rice Events
作 者：
ZHANG Mingzhe;ZHANG Xiaofeng;XU Lili;CHEN Xiaomei;ZHOU Yuan;YIN Wenxiu;CHEN Wujian;LI Xiaojun;Zhejiang Academy of Science and Technology for Inspection and Quarantine;Zhoushan Entry-Exit Inspection and Quarantine Bureau;
关键词：
multiplex PCR;;capillary electrophoresis;;genetic modified rice;;identification of genetically modified events
摘 要：
In this research, a high-throughput and specific method for the detection of genetically modified(GM) rice events was developed using fluorescence multiplex PCR and capillary electrophoresis based on fluorescence-labeled primers for endogenous, exogenous and event-specific genes. The fluorescence multiplex PCR method could specifically detect four GM rice events with a sensitivity of 0.1%. This method has the potential to be applied in the inspection and quarantine of import and export genetically modified commodities and in food safety detection.
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