当前位置: 首页 > 文章 > 生长调节剂对白鲜愈伤组织的诱导及药用成分含量影响 延边大学农学学报 2019 (4) 55-61
Position: Home > Articles > Effects of the growth regulator on inducing callus and medicinal ingredients in Dictamnus dasycarpus Agricultural Science Journal of Yanbian University 2019 (4) 55-61

生长调节剂对白鲜愈伤组织的诱导及药用成分含量影响

作  者:
王馨琪;贾晓龙;刘卉;李美阳;南桂仙
单  位:
延边大学农学院
关键词:
白鲜;愈伤组织;白鲜碱;黄柏酮;梣酮
摘  要:
为筛选白鲜愈伤组织培养的适宜培养基,试验以延边地区野生白鲜种子为材料,研究了MS培养基中植物生长调节剂种类及其浓度对愈伤组织诱导和生长以及愈伤组织中白鲜碱、黄柏酮和梣酮积累的影响。结果表明:在MS+2.0 mg/L NAA+2.0 mg/L 6-BA以及MS+2.0 mg/L 2,4-D+2.0 mg/L 6-BA培养基中,愈伤组织诱导率及生长较好;在MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA培养的愈伤组织中,白鲜碱和梣酮的有效积累量最高,在MS+2.0 mg/L 2,4-D+2.0 mg/L 6-BA培养的愈伤组织中,黄柏酮的有效积累量最高。
译  名:
Effects of the growth regulator on inducing callus and medicinal ingredients in Dictamnus dasycarpus
作  者:
WAGN Xinqi;JIA Xiaolong;LIU Hui;LI Meiyang;NAN Guixian;Agricultural College of Yanbian University;
关键词:
Dictamnus dasycarpus;;callus;;dictamnine;;obacunone;;fraxinellone
摘  要:
In orther to screen the suitable medium for callus culture, seeds of wild Dictamnus dasycarpus growing in Yanbian area were used as materials in this experiment, the effects of plant growth regulators and their concentrations in MS medium on inducing and growth of callus,and accumulation of dictamnine, obacunone and fraxinellone in callus were investigated. The results showed that the callus grew well in the MS+2.0 mg/L NAA+2.0 mg/L 6-BA, and MS+2.0 mg/L 2,4-D+2.0 mg/L 6-BA medium. The effective accumulation of dictamnine and fraxinellone in callus on the MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA medium, and obacunone in callus on the MS+2.0 mg/L 2,4-D+2.0 mg/L 6-BA medium were the highest.

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