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Position: Home > Articles > A Rapid Method of Genomic DNA Extraction of Gingko Agricultural Science & Technology and Equipment 2014 (5) 17-18

一种银杏叶片DNA快速提取方法

作  者:
姜力耘;邵屹;王战男;郭铁丰;王博
单  位:
辽宁省经济林研究所;国有黑山县机械林场
关键词:
DNA提取;银杏叶片;RAPD;PCR
摘  要:
在CTAB法的基础上,建立适于银杏叶片DNA微量快速提取的新方法。经紫外分光光度和琼脂糖凝胶电泳检测,该方法提取的DNA在质量和产量上都可满足后续扩增反应的要求。与常规CTAB方法相比,该方法操作简单、速度快、时间短。
译  名:
A Rapid Method of Genomic DNA Extraction of Gingko
作  者:
JIANG Liyun;SHAO Yi;WANG Zhannan;GUO Tiefeng;WANG Bo;Liaoning Institute of Economic Forestry;State-owned Machinery Forest Farm of Heishan County;
关键词:
DNA extraction;;gingko;;RAPD;;PCR
摘  要:
A method of rapid DNA extraction from gingko was established based on CTAB procedure. Utraviolet spectrophotometer and agarose gel electrophoresis methods were used to test the DNA quality. The DNA extracted with this method can meet the requirement for the follow-up amplified reaction both in terms of quality and quantity. Compared with CTAB procedure, this method is simpler, faster and more costs-saving.

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