Position: Home > Articles > Estimation of Genomic Size and Physical Location of rDNA of Chrysanthemum weyrichii
Northern Horticulture
2016
(12)
101-105
Chrysanthemum weyrichii基因组测定及rDNA定位
作 者：
钱双;李昂;赵鑫;杨树华;李名扬;葛红
单 位：
中国农业科学院蔬菜花卉研究所;西南大学园艺园林学院
关键词：
Chrysanthemum weyrichii;流式细胞术;染色体;rDNA;荧光原位杂交
摘 要：
以Chrysanthemum weyrichii为试材,采用流式细胞术估算其基因组大小,同时利用染色体计数法统计了C.weyrichii的染色体条数并对其rDNA染色体定位进行了研究。结果表明:C.weyrichii基因组大小为10.02Gb,染色条数为2n=8x=72,5S rDNA杂交信号有4对位点,位于染色体的亚中部;45S rDNA杂交信号有8对位点,位于染色体的末端。该研究结果为该物种基因组分析以及菊属植物染色体进化研究提供了依据。
译 名：
Estimation of Genomic Size and Physical Location of rDNA of Chrysanthemum weyrichii
作 者：
QIAN Shuang;LI Ang;ZHAO Xin;YANG Shuhua;LI Mingyang;GE Hong;College of Horticulture and Landscape Architecture,Chongqing Engineering Research Center for Floriculture,Southwest University;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,National Flowers Improvement Center of China;
关键词：
Chrysanthemum weyrichii;;flow cytometry;;chromosome;;rDNA;;fluorescence in situ hybridization
摘 要：
Taking Chrysanthemum weyrichii as test material,the genome size of C.weyrichii was estimated by flow cytometry.The chromosome numbers and rDNA chromosomal location of C.weyrichii were studied by chromosome counting method and fluorescence in situ hybridization for the first time.The results showed that the genome size was 10.02 Gb,chromosome numbers were 2n=8x=72,four pairs of 5S rDNA located at submetacentric regions and eight pairs of 45S rDNA located at terminal regions were detected.This study provided a valuable basis for future genomic and cytogenetic studies in C.weyrichii.
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