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Position: Home > Articles > RP-HPLC Analysis of d1-Tetrahydropalmatine and Protopine in Corydalis yanhusuo Produced from Shaanxi Journal of Anhui Agricultural Sciences 2009,37 (21) 18-19+26

高效液相法测定陕西产延胡索中延胡索乙素、原阿片碱的含量

作  者:
冯自立;周建军;张涛;张晓娟;季晓辉
单  位:
陕西理工学院陕西省资源生物重点实验室;陕西理工学院化学与环境科学学院
关键词:
高效液相色谱法;延胡索乙素;原阿片碱;延胡索
摘  要:
[目的]测定陕西产延胡索中延胡索乙素、原阿片碱的含量。[方法]采用HPLC法,色谱柱为AgilentODSC18色谱柱(150 mm×4.6mm,5μm),流动相为0.1%磷酸缓冲液-甲醇(65∶35,三乙胺调pH值至6.0),检测波长为280 nm,流速为1.0 m l/m in,柱温为25℃,进样量10μl。[结果]陕西产延胡索中延胡索乙素、原阿片碱的平均含量分别为0.977和0.651 mg/g,2种成分平均加样回收率分别为98.02%(RSD=1.87%)和98.63%(RSD=1.43%)。[结论]陕西产延胡索中延胡索乙素、原阿片碱含量与其他文献资料相比较高,符合2005版药典含量要求。
译  名:
RP-HPLC Analysis of d1-Tetrahydropalmatine and Protopine in Corydalis yanhusuo Produced from Shaanxi
作  者:
FENG Zi-li et al (Shaanxi University of Technology,Shaanxi Provincial Key Laboratory of Bio-resource,Hanzhong,Shaanxi 723001)
关键词:
HPLC;d1-Tetrahydropalmatine;Protopine;Corydalis yanhusuo
摘  要:
[Objective]The research aimed to establish a method for determination of d1-Tetrahydropalmatine and protopine in Corydalis yanhusuo.[Method]HPLC separation was carried on a column of ODS(4.6 mm×150 mm,5 μm),with the mobile phase of 0.1% phosphate buffer-methanol(65∶35) at the flow rate of 1.0 ml/min,and the detection wavelength was set at 280 nm.[Result]The average content of d1-Tetrahydropalmatine and protopine in Corydalis yanhusuo produced from was 0.977 and 0.651 mg/g respectively.Their average recovery were 98.02%(RSD=1.87%) and 98.63%(RSD=1.43%).[Conclusion]Compared with other literatures,the content of two active components in Corydalis yanhusuo W.T.Wang from Shaanxi,the content of d1-Tetrahydropalmatine and protopine were relative higher,which could meet the content requirements of China Pharmacopeia(2005).

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