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当前位置: 首页 > 文章 > 基于籽粒贮藏蛋白遗传多样性的小麦条形码研究 麦类作物学报 2012,32 (4) 32-37
Position: Home > Articles > Study on Bar-coding of Wheat Variety Based on Genetic Diversity of Seed Storage Protein Journal of Triticeae Crops 2012,32 (4) 32-37

基于籽粒贮藏蛋白遗传多样性的小麦条形码研究

作  者：
康志钰;王建军
单  位：
云南农业大学农学与生物技术学院
关键词：
小麦;籽粒;高分子量麦谷蛋白亚基;醇溶蛋白;条形码
摘  要：
为便于小麦品种管理及保护,针对植物DNA条形码研制存在的问题,以36份品种为材料,分析其HMW-GS和醇溶蛋白组分,并根据谱带的有无,对谱带进行数量化处理,存在的谱带标为1,不存在的谱带标为0,建立谱带二进制代码,再转化为十进制代码,最后通过数据整合,建立了小麦品种身份识别码,并将其转换为条形码,研制出基于籽粒贮藏蛋白遗传多样性的小麦身份识别码制作方法,使原来需要用119位数字表明的品种间差距现在只需37位数字即可表示出来。
译  名：
Study on Bar-coding of Wheat Variety Based on Genetic Diversity of Seed Storage Protein
作  者：
KANG Zhi-yu,WANG Jian-jun(Agronomy and Biotechnology College,Yunnan Agricultural University,Kunming,Yunnan 650201,China)
关键词：
Wheat;HMW-GS;Gliadin;Identification code;Bar-coding
摘  要：
To be convenient for the management and protection of wheat varieties,and aimed at the problems on DNA bar-coding of plant,the high molecular weight gluten subunit(HMW-GS) and gliadin of 36 wheat varieties were investigated and used to establish their codes.By number processing,according to the presence and absence of the bands as presence of band was signed with "1" and absence of band was signed with "0",the binary code system was established and then the binary code system was translated into decimal code system,finally,the identification code system of wheat variety was established through the conformity of data,and translated the identification code for bar-coding.An method for the identification code system of wheat was built based on the genetic diversity of seed storage protein.In this way,the difference between the varieties could be distinguished by 37 digits,instead of 119 digits used in the past.

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