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“三峡虹”红阳猕猴桃组织培养体系的筛选

作  者:
甘丽萍;阮神清;曾晓琳
单  位:
重庆三峡学院生命科学与工程学院
关键词:
红阳猕猴桃;组织培养;愈伤组织
摘  要:
以红阳猕猴桃叶片、嫩茎、花蕾为外植体,MS为基本培养基,设置不同外植体消毒方法、不同的激素配方进行组织培养,比较了不同消毒剂对外植体的消毒效果、愈伤组织、不定芽的诱导情况,以及形成愈伤组织能力较强的外植体种类。结果表明:用75%酒精处理外植体30s,再以1%NaClO消毒10min,消毒效果达75.0%;诱导愈伤组织最适培养基为MS+1.0mg/L ZT+0.1mg/L NAA;诱导不定芽最适培养基为MS+1.0mg/L 6-BA+0.1mg/L NAA;用嫩茎作为外植体,诱导愈伤组织效果最好。
译  名:
Tissue Culture System Screening of Actinidia chinensis cv.Hongyang‘Sanxia Horg'
作  者:
GAN Liping;RUAN Shenqing;ZENG Xiaolin;College of Life Science and Engineering,Chongqing Three Gorges University;
关键词:
Actinidia chinensis cv;;Hongyang;;tissue culture;;callus
摘  要:
Taking the leaf,stems,buds of Hongyang kiwi fruit as explants,MS as basic medium,the effect of different disinfection methods,callus and bud induction ability of hormone formulations was studied.The results showed that the disinfectant method of 75% alcohol treatment explants 30 s,then 1% NaClO disinfection 10 min was the best with sterilizing effect reached 75.0%;callus optimum medium was MS+1.0mg/L ZT+0.1mg/L NAA while optimum medium for inducing adventitious buds was MS+1.0mg/L 6-BA+0.1mg/L NAA.The stems was the best explants for callus formation.

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