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当前位置: 首页 > 文章 > Nesfatin-1调控斜带石斑鱼生殖作用的研究 渔业研究 2016,38 (1) 5-13
Position: Home > Articles > The study of nesfatin-1 in Epinephelus coioides reproductive regulation Journal of Fisheries Research 2016,38 (1) 5-13

Nesfatin-1调控斜带石斑鱼生殖作用的研究

作  者：
王庆;刘琼瑜;李益祺;刘雅莉;李玉;陈嘉兴;张海发;李水生;张勇
单  位：
中山大学水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室;广东省海洋渔业实验中心
关键词：
斜带石斑鱼;nesfatin-1;组织分布;生殖调控
摘  要：
Nesfatin-1是核连蛋白-2(nucleobindin-2,NUCB2)裂解后产生的一种神经肽。本研究克隆得到斜带石斑鱼两种编码NUCB2(NUCB2a和NUCB2b)基因的c DNA序列(nesfatin-1a和nesfatin-1b),并对它们的表达模式进行了研究。结果表明nesfatin-1a和nesfatin-1b在脑组织中广泛表达。在下丘脑中,两种nesfatin-1在卵巢发育的Ⅰ-Ⅴ时期表达量都比较低,在Ⅵ时期卵巢(退化的卵黄生成阶段的卵母细胞)中的表达量显著升高,揭示nesfatin-1可能参与了卵巢成熟的调控。进一步采用腹腔注射实验研究了两种nesfatin-1对生殖轴的影响。注射nesfatin-1a和nesfatin-1b 15 min和60 min后,下丘脑中促性腺激素释放激素Ⅰ型(Gn RH-Ⅰ)和促性腺激素释放激素Ⅲ型(Gn RH-Ⅲ)的表达量都显著下降;此外,垂体中FSH-β的表达在注射nesfatin-1 60 min后显著下调。这些结果表明nesfatin-1a和nesfatin-1b参与了斜带石斑鱼下丘脑-垂体-性腺轴(HPG)的调控。
译  名：
The study of nesfatin-1 in Epinephelus coioides reproductive regulation
作  者：
WANG Qing;LIU Qiongyu;LI Yiqi;LIU Yali;LI Yu;CHEN Jiaxing;ZHANG Haifa;LI Shuisheng;ZHANG Yong;LIN Haoran;Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals,Sun Yat-Sen University;Marine Fisheries Development Center of Guangdong Province;College of Ocean,Hainan University;
关键词：
Epinephelus coioides;;nesfatin-1;;tissue distribution;;reproductive regulation
摘  要：
Nesfatin-1 is the hypothalamus gene encoding nuclear protein-2(nucleobindin-2,NUCB2) produces a neuropeptide after cracking.Studies have shown that nesfatin-1 play an important role in reproductive regulation,in order to study the nesfatin-1 relation with Epinephelus coioides reproductive regulation,in this paper,we have cloned two NUCB2(NUCB2 a and NUCB2b) gene,analyzes NUCB2 coding protein get sequence of amino acids about two nesfatin-1(nesfatin-1a and nesfatin-1b),at the same time,we analysis of the two kinds of nesfatin-1's signal peptide and mature peptide,found it is consistent with that of vertebrates.Through real-time quantitative PCR technology,we determined the m RNA expression level of Epinephelus coioides nesfatin-1 in different tissues.The expression patterns revealed that,nesfatin-1a and nesfatin-1b widely expressed in the brain tissue,especially nesfatin-1b abundantly expressed in the pituitary,also has the corresponding expression in the gonads.We determined thenesfatin-1 m RNA expression level of hypothalamus during ovary development,the result reveal that,nesfatin-1a and nesfatin-1b in the ovaries Ⅵperiod(degradation of yolk formation stage oocytes) significantly increased,showed that two kinds of nesfatin-1 are likely to participate in the regulation of ovarian mature.The intraperitoneal injection experiment results show that,injecting nesfatin-1a and nesfatin-1b 15 min,both Gn RH1 and Gn RH3 m RNA expression level of the hypothalamus are reduce,but in the pituitary LH-βand FSH-βm RNA expression level has not changed.Injection of nesfatin-1a and nesfatin-1b 60 min,the m RNA expression level of the hypothalamus Gn RH1 and Gn RH3 still suppressed.Injected nesfatin-1a and nesfatin-1b after 60 min,there have no effect change on the expression level of LH-β,but significantly reduced the expression of FSH-β.Through the above study shows nesfatin-1a and nesfatin-1b involved in Epinephelus coioides reproductive regulation.

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