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Position: Home > Articles > Depletion of high-abundance plasma proteins from Chinese shrimp Fenneropenaeus chinensis for proteomics analysis by ultracentrifugation Journal of Fishery Sciences of China 2010,17 (4) 695-700

中国明对虾血浆蛋白质组学研究中高丰度蛋白的超速离心去除技术

作  者:
王宝杰;刘梅;蒋克勇;张国范;王雷
单  位:
中国科学院海洋研究所
关键词:
中国明对虾;血浆;蛋白质组;双向电泳;高丰度蛋白去除;血蓝蛋白;超速离心
摘  要:
应用超速离心技术对中国明对虾(Fenneropenaeus chinensis)血浆样品进行处理,比较去除和未去除高丰度蛋白血浆的双向凝胶电泳(2-DE)图谱。结果表明:对虾血浆高丰度蛋白经去除后,血浆样品中的低丰度蛋白得到较好的分离和浓缩。未处理样品的2-DE图谱分离的蛋白质170个,而去除高丰度蛋白后血浆2-DE图谱上分辨率增高,分离出蛋白质点520个,一些低丰度蛋白质被有效分离出来。研究证实,超速离心技术可有效去除对虾血浆高丰度蛋白(血蓝蛋白),提高低丰度蛋白的检出敏感性,为对虾等甲壳动物的血浆蛋白质组学研究提供了基础保障。
译  名:
Depletion of high-abundance plasma proteins from Chinese shrimp Fenneropenaeus chinensis for proteomics analysis by ultracentrifugation
作  者:
WANG Baojie 1,2 ,LIU Mei 1 ,JIANG Keyong 1 ,ZHANG Guofan 1, WANG Lei 1 Institute of Oceanology,the Chinese Academy of Sciences,Qingdao 266071,China;2.Graduate School of Chinese Academy of Sciences,Beijing 100039,China)
关键词:
Fenneropenaeus chinensis;plasma;proteomics;2-DE;high abundance proteins depletion;hemocyanin; ultracentrifugation
摘  要:
To establish the method for removing high-abundance proteins in shrimp plasma proteomics analysis, depletion of plasma high-abundance proteins,such as hemocyanins,was practiced by ultracentrifugation. The efficiency of the method was assessed by 2-DE. The ultracentrifuge method for removing plasma high-abundance proteins was established to remove most hemocyanins. The proteins spots of 2-DE map were more clear and some low-abundance proteins were visualization after ultracentrifugation. The established methods for high-abundance depletion can be effectively applied in shrimp and other crustaceans plasma proteomic study.

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