Lv Xixi;Feng Shikun;College of Fisheries, Henan Normal University;

Figla;;Cyprinus carpio;;expression

Germline factor a(figla), a specific transcription factor for female germ cells, has been proved to have a regulatory effect on the development of mammalian follicles and the formation of zona pellucida. Abnormal figla gene can cause premature ovarian failure. However, the potential role of figla in teleosts remains elusive. In order to determine the potential role of figla in the ovary development of yellow river carp(Cyprinus carpio), the full length ORF of figla gene was isolated and the expression of it in different tissues and embryonic development stages was analyzed by RT-PCR in Cyprinus carpio. The results showed that the ORF of figla was 672 bp in length, encoding 223 amino acids. Amino acid sequence comparison analysis showed that the figla homology of Cyprinus carpio and other species was low. Alignment of the Cyprinus carpio Figla with those from other organisms revealed a low identity. Phylogenetic analysis showed that the figla of Cyprinus carpio clustered with those of fish.RT-PCR analysis revealed that the figla gene was highly expressed in the ovary of Cyprinus carpio, but was not detected in other tissues(including males'). In the different periods during the early stages of development figla was only detected in the unfertilized eggs, fertilized egg and cleavage stage. Taken together, as a maternal factor,figla may play a regulatory role in ovarian development in Cyprinus carpio.