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当前位置: 首页 > 文章 > 稀有鮈鲫生命早期的己烯雌酚暴露对生长发育与繁殖的影响 水生生物学报 2005,29 (6)
Position: Home > Articles > EFFECTS OF DIETHYLSTILBESTROL EXPOSURE IN EARLY LIFE STAGE ON DEVELOPMENT AND REPRODUCTION IN RARE MINNOW,GOBIOCYPRIS RARUS Acta Hydrobiologica Sinica 2005,29 (6)

稀有鮈鲫生命早期的己烯雌酚暴露对生长发育与繁殖的影响

作  者：
钟雪萍;徐盈;梁勇;廖涛;王剑伟
单  位：
中国科学院水生生物研究所淡水生态与生物技术国家重点实验室
关键词：
稀有鮈鲫;己烯雌酚;生命早期;内分泌干扰物
摘  要：
研究了己烯雌酚(DES)对稀有鲫(Gobiocypris rarus)生命早期暴露的影响。经10μg/L和100μg/L己烯雌酚暴露26d后,稀有鲫死亡率升高,生长发育迟缓,鱼体内卵黄蛋白原(VTG)的诱导明显。经过一段时间清水养殖后,暴露组中雌鱼比例增加,雌雄鱼生长较对照组有显著变化。雌鱼性腺发育及产卵量与对照组相比虽无显著差异,但暴露组成鱼所繁育后代与对照组相比受精率、孵化率显著下降,死亡率、畸形率明显上升。这些结果说明己烯雌酚生命早期暴露影响稀有鲫的生长发育及生殖,稀有鲫生命早期暴露实验可以用于评价水生态系统中内分泌干扰物的生态影响。
译  名：
EFFECTS OF DIETHYLSTILBESTROL EXPOSURE IN EARLY LIFE STAGE ON DEVELOPMENT AND REPRODUCTION IN RARE MINNOW,GOBIOCYPRIS RARUS
作  者：
ZHONG Xue-Ping,XU Ying,LIANG Yong,LIAO Tao and WANG Jian-Wei(Institute of Hydrobiology,the Chinese Academy of Sciences;State Key Laboratory of Freshwater Ecology and Biotechnology,Wuhan 430072)
关键词：
Diethylstilbestrol;Endocrine-disrupting chemicals;Early life stage;Rare minnow
摘  要：
Rare minnow,Gobiocypris rarus, is a Chinese cyprinid.It has many attractive features that make it a suitable organism in aquatic toxicity tests,including small size,wide temperature range, easily being cultured in laboratory,large amount of eggs and short life cycle. It has also been reported this species is susceptible to heavy metals and other xenobiotics.However,little has been known about the endocrine disruptions of xenoestrogens in this native Chinese species.The aim of the study was to investigate the effects of diethylstilbestrol(DES) exposure in early life stage on development and reproduction of rare minnow.Rare minnow were raised in the laboratory.They were maintained in a light/dark cycle of 14∶10 h at 23-26℃ and fed once a day.To start the test with DES,embryos(<8 h postfertilization) were collected from at least three spawnings as required by standard protocol.Based on a 28 days preliminary exposure study,nominal concentrations of DES at 10 and 100μg/L were selected for this study.Rare minnow were exposed to these two concentrations of DES from newly fertilized embryos to 23 days posthatch.The mortality,abnormal behavior and appearance of the larvae were recorded during the exposure.At the end of exposure.fish were sampled to measure body weight,body length and then stored at-80℃for VTG analysis and Total T_4(TT_4)measurement.At 24 days posthatch,rare minnow were transferred to clean water.At 175 days posthatch,15 fish were sampled to measure body weight,body length and then stored at-80℃for Vtg analysis.Four mating pairs were selected from control group and DES-exposed groups for examination of fecundity and fertility. At the end of exposure,it was found that exposure to DES caused an increase in mortality and vitellogenin(VTG)levels.Growth was significantly inhibited in the fish exposed to DES.After a five-month period culture in clean water,the sex ratio(male:female)observed was changed from 46:54 in controls to 31:69 at 10μg/L DES and 33:67 at 100μg/L DES.Reproductive capability was depreciated in the DES-treated fish as measured by fertility of eggs,hatchability of eggs and survival of F_1 larva.The growth and VTG level had a significant change in the DES-treated fish when compared to the control group.The results indicate that exposure to DES in early life stage can cause adverse effects on development and reproduction.It suggests that rare minnow is suitable for early life stage test in assessing the environmental impact of endocrine-disrupting chemicals.

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