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当前位置: 首页 > 文章 > 动物源产CMY-2大肠杆菌分子流行病学研究 中国畜牧兽医 2013,40 (10) 47-51
Position: Home > Articles > Epidemiology Investigation of CMY-2-producing E.coli from Animals China Animal Husbandry & Veterinary Medicine 2013,40 (10) 47-51

动物源产CMY-2大肠杆菌分子流行病学研究

作  者：
郭玉芳;赵秋云;姜芮;任金程;杨玲;甄盼盼;汤电;蒋红霞
单  位：
河北新华科极兽药有限公司;武汉科前动物生物制品有限责任公司;华南农业大学兽医学院
关键词：
大肠杆菌;耐药性;动物源;CMY-2
摘  要：
为调查产CMY-2大肠杆菌在广东各养殖场的流行情况,对2010—2011年间分离自猪、鸡、鸭、鹅等动物的1293株大肠杆菌,采用PCR方法筛选出blaCMY-2阳性菌株,琼脂稀释法测定阳性菌株对17种抗微生物药物的敏感性;接合转移试验和XbaⅠ酶切PFGE图谱分析blaCMY-2基因转移扩散的方式。结果显示,1293株大肠杆菌中27株含有blaCMY-2基因,检出率为2.09%,均为多重耐药菌株,主要耐药谱型为AMP/CHL/TET/FLF/CTF/CTX/CAZ/CTR/GEN/CIP/ENR/NAL/OQX;27株携带blaCMY-2基因菌株中有14株的blaCMY-2基因可随质粒转移到受体菌E.coli C600中,且往往与blaTEM-1和(或)qnrS1共同转移;PFGE分析结果显示,27株携带blaCMY-2基因菌株共产生17条谱带,其中有4株菌株,两两分别来自同一地区,存在克隆传播关系。提示,在广东地区食品动物养殖场内存在产CMY-2大肠杆菌的克隆传播,且blaCMY-2基因伴随可转移质粒或其他可转移移动元件可能是造成产CMY-2大肠杆菌流行分布的主要原因。
译  名：
Epidemiology Investigation of CMY-2-producing E.coli from Animals
作  者：
GUO Yu-fang;ZHAO Qiu-yun;JIANG Rui;REN Jin-cheng;YANG Ling;ZHEN Pan-pan;TANG Dian;JIANG Hong-xia;Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation,College of Veterinary Medicine,South China Agricultural University;Hebei Xinhua Keji Animal Medicine Co.,Ltd.;Wuhan Keqian Animal Biological Products Co.,Ltd.;
关键词：
E.coli;;resistance;;animals;;CMY-2
摘  要：
To investigate the epidemiology of CMY-2-producing E.coli from different animals throughout Guangdong province,1293 isolates from pig,chicken,duck and goose from 2010 to 2011 were recruited in this study.All the isolates were screened for blaCMY-2 gene by PCR and sequenced;the MICs of blaCMY-2 -positive isolates to 17 kinds of antimicrobial drugs were tested by agar dilution;conjugation experiments were performed on blaCMY-2 harboring isolates to explore the transferability of blaCMY-2 gene to E.coli C600, and XbaⅠ-PFGE analysis were used to determine the transfer and spread manner of blaCMY-2 among E.coli isolates.The results showed that 27(2.09% )of 1293 isolates carried blaCMY-2 , and were present multi-resistant phenotypes,in which the main one was AMP/CHL/TET/FLF/CTF/CTX/CAZ/CTR/GEN/CIP/ENR/NAL/OQX.The blaCMY-2 gene in 14 of 27isolates transferred successfully to E.coli C600 by plasmids, and most of them co-transferred with blaTEM-1 and /or qnrS1.PFGE profiles showed that 17different patterns were successfully gotten, and two from the same place were clonally related as well as another two isolates.CMY-2 encoding gene on a transferability plasmid or carried by other mobile element presumably was the major cause for the epidemiology of CMY-2-producing E.coli among food animals.

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