当前位置: 首页 > 文章 > 薄层层析-分光光度法检测纤毛虫中的卵磷脂 湖北农业科学 2012,51 (22) 5185-5187
Position: Home > Articles > Determination of Phosphatidylcholine in Protozoa by Thin Layer Chromatography-Spectroscopy Hubei Agricultural Sciences 2012,51 (22) 5185-5187

薄层层析-分光光度法检测纤毛虫中的卵磷脂

作  者:
盛东峰;孙明俊
单  位:
周口师范学院生命科学系;周口中食富民牧业有限公司
关键词:
纤毛虫;卵磷脂;薄层层析;分光光度法
摘  要:
卵磷脂(PC)在纤毛虫中含量比较稳定,通过纤毛虫PC含量来估计出纤毛虫的量。采用薄层层析-分光光度法测定了山羊瘤胃纤毛虫中PC的含量,结果表明山羊瘤胃纤毛虫PC含量为17.9~24.1 mg/g。该法简便、快速、有效,可作为纤毛虫卵磷脂含量的检测方法。
译  名:
Determination of Phosphatidylcholine in Protozoa by Thin Layer Chromatography-Spectroscopy
作  者:
SHENG Dong-feng1,SUN Ming-jun2(1.Department of Life Science,Zhoukou Normal University,Zhoukou 466001,Henan,China;2.Zhoukou Fumin Animal Husbandry Limited Company,Zhoukou 466001,Henan,China)
关键词:
protozoa;phosphatidylcholine;thin layer chromatography;spectroscopy
摘  要:
Phosphatidylcholine(PC) is a special stable marker in the protozoa,the content of which could be used as an indicator for evaluating the quantity of protozoa.Thin layer chromatography-spectroscopy was established to determine the content of PC in the protozoa.The results showed that the content of phosphatidylcholine in protozoa was in the range of 17.8~ 24.2 mg/g.In conclusion,this method was simple,rapid and efficient.It can be used for determining the relative content of phosphatidylcholine in protozoa.

相似文章

计量
文章访问数: 20
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊