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当前位置: 首页 > 文章 > 降解玉米秸秆纤维素复合菌的选育及其酶活研究 新疆农业科学 2015,52 (4) 712-717
Position: Home > Articles > Degradation of Corn Straw Stalk Fiber Composite Bacteria Breeding and Its Enzyme Activity Xinjiang Agricultural Sciences 2015,52 (4) 712-717

降解玉米秸秆纤维素复合菌的选育及其酶活研究

作  者：
张二红;潘晓亮;王新峰;赖汉卿;蒋立斌;郭腾飞
单  位：
石河子大学动物科技学院
关键词：
纤维素酶;筛选;纤维素降解菌
摘  要：
【目的】提高玉米秸秆纤维素的降解率。【方法】从腐烂的秸秆、森林土及羊瘤胃液等富含纤维素分解菌的样品中筛选出降解纤维素的菌株。样品以玉米秸秆为碳源富集培养后,采用刚果红纤维素琼脂平板法初步筛选纤维素降解菌,再以CMCase(羧甲基纤维素酶)酶活性为指标进行复筛,对复筛获得的高效菌株进行组合培养,筛选出高效组合菌群,进行菌株鉴定。【结果】筛选获得了3株活性较高的纤维素分解菌,通过形态及16S r DNA序列分析对其进行种属鉴定N05、N13为枯草芽孢杆菌,N21为黑曲霉;并对其进行组合培养,得到1个较好组合NSS,其CMC酶活性为6.07 U/m L,比单菌株有一定程度提高。【结论】混合菌群的酶活优于单一菌株。
译  名：
Degradation of Corn Straw Stalk Fiber Composite Bacteria Breeding and Its Enzyme Activity
作  者：
ZHANG Er-hong;PAN Xiao-liang;WANG Xin-feng;LAI Han-qing;JIANG Li-bin;GUO Teng-fei;College of Animal Science and Technology,Shihezi University;
关键词：
cellulose;;screening;;cellulose degradative strain
摘  要：
【Objective】T0 increase the rate of the degradation of corn straw stalk fiber. 【Method】Degradation of cellulose decomposing bacteria strains were screened from decaying straws,forest soil and sheep rumen fluid rich in cellulose samples,cellulose Congo-red culture medium was adopted to screen samples,and then CMC( carboxymethyl cellulase) activity was used to re-screen,by which the strains with high capacity were obtained. The strains were mixed and a microbial community with high capacity was screened. 【Result】The results showed that three strains that could decompose cellulose better were obtained from the samples.Then they were cultured by mixed fermentation. The combination of N5N13N21 enhanced cellulose CMCase activities to some degree,and the activity of CMCase was 60. 76 U/ml. The strains were judged by the characteristics of the colony and 16 S r DNA sequence analysis on the species. The strains of N05 and N13were bacillussubtilis,and N21 was aspergillus niger. 【Conclusion】The study found that the cellulose activity of the mixed bacteria was superior to a single strain.

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