Comparison of Two Methods in Measuring Outbred Wuzhishan Minipig′s Mandible

MIN Fan-gui1,2,PAN Jin-chun1,YUAN Wen1,LUO Xian-li1,WANG Xi-long1(1.Guangdong Laboratory Animal Monitoring Institute,Guangzhou,Guangdong 510260,China;2.Guangdong Medical College,Zhanjiang,Guangdong 524023,China)

outbred Wuzhishan mini-pig;mandible;direct method;indirect method

To compare the speciality of mandible variables between the direct and indirect methods and evaluate validity of two methods preliminarily,25 mandibles of Wuzhishan minipigs(WZSP)(17 females and 8 males) between 6 to 12 months old were chosen to determine the variables.The direct method chosed 23 variables determined by the universal protractor,vernier caliper and band tape,while the indirect method chosed 11 variables on the photo taken by digital camera and the variables were determined by Photoshop soft.Between the females and males,6 out of 23 variables in direct method and 5 out of 11 variables in indirect method have significant difference(P<0.05).6 variables in direct method and 7 variables in indirect method were introduced into their own linear discriminant functions of stepwise discriminant method.However the indirect method showed higher accuracy than the direct method in resubstitution summary using linear discriminant function.It could be concluded that two methods could distinguish the sex in resubstitution summary using linear discriminant function,but the variables of indirect method were more special for distinguishing sex of WZSP.The application of mandible variables in genetic testing yet should be confirmed in a broader context.