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当前位置: 首页 > 文章 > 雨生红球藻诱变后生长速率和超微结构的变化 核农学报 2015,29 (4) 623-628
Position: Home > Articles > Changes in Growth Rate and Ultrastructure of Haematococcus pluvialis After Mutagenesis Journal of Nuclear Agricultural Sciences 2015,29 (4) 623-628

雨生红球藻诱变后生长速率和超微结构的变化

作  者：
沈国明;牛健康;陆开形;尚宏芹
单  位：
菏泽学院生命科学系/植物生物学重点实验室;宁波大学科学技术学院
关键词：
生长率;雨生红球藻;致死率;亚硝基胍;色素含量;超微结构
摘  要：
为探讨亚硝基胍(N-methyl-N'-Nitro-N-Nitrosoguanidine,NTG)对雨生红球藻(Haematococcus pluvialis)生长速率及超微结构变化的影响,用不同浓度的NTG处理藻类,对藻类的生长速率、色素含量和超微结构的变化进行了研究。结果表明:即使低浓度(0.5 g·L-1)的NTG对雨生红球藻也是致死的,致死率与NTG浓度呈正相关。藻类经中等浓度(2.5 g·L-1)NTG诱变后,存活藻类的增长速率K值和虾青素含量分别达到0.381±0.0289和2.9±0.29 mg·L-1。低浓度NTG处理藻类其叶绿素含量略有上升,但高浓度(3.5 g·L-1)时叶绿素含量明显减少。低NTG浓度处理时藻类细胞结构变化不大,但高浓度处理时细胞膜、线粒体及叶绿体等均遭受不同程度的损伤。本研究可为深入理解NTG诱变藻类的生理生化机制提供借鉴。
译  名：
Changes in Growth Rate and Ultrastructure of Haematococcus pluvialis After Mutagenesis
作  者：
SHEN Guoming;NIU Jiankang;LU Kaixing;SHANG Hongqin;Key laboratory of Plant Biology / Department of Life Sciences Heze University;Faculty of Life Sciences and Biotechnology,Ningbo University;
关键词：
growth rate,Haematococcus pluvialis,lethal rate,N-methyl-N'-Nitro-N-Nitrosoguanidine,pigment content,ultrastructure
摘  要：
To investigate the influcences of N- methyl- N'- Nitro- N- Nitrosoguanidine( NTG) on growth rate and ultrastructure of H. pluvialis( Haematococcus pluvialis),the growth rate,pigments content and ultrastructure were investigated after treated by NTG at different concentrations. The results showed that even a low concentration( 0. 5g·L- 1) of NTG was lethal for H. pluvialis and the lethality rate was related positively to NTG concentration. After mutated by medium concentration( 2. 5g·L- 1) of NTG,the growth rate( K value) and astaxanthin amount of survival algae reached up to 0. 381 ± 0. 0289 and 2. 9 ± 0. 29 mg·L- 1,respectively. The chlorophyll content increased slightly under a low NTG concentration but reduced with a high concentration. With low concentration of NTG,there were no changes in cell structure,but some organelles,such as cell membrane,mitochondria or chloroplast were damaged after treatment with high concentration of NTG( 3. 5 g L- 1). This study provided insights for further understanding of the physiological and biochemical mechanisms of algae mutation by NTG.

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