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当前位置: 首页 > 文章 > 花粉介导的转Bt基因棉花田间基因流监测 应用生态学报 2007,18 (4) 801-806
Position: Home > Articles > Monitoring of pollen-mediated gene flow from transgenic Bt cotton Chinese Journal of Applied Ecology 2007,18 (4) 801-806

花粉介导的转Bt基因棉花田间基因流监测

作  者：
王长永;刘燕;周骏;陈建群;钦佩
单  位：
南京大学生命科学学院;国家环境保护总局南京环境科学研究所
关键词：
转Bt基因棉花;基因流;花粉漂移;花粉粒染色法;监测
摘  要：
采用花粉粒染色法对转Bt基因棉的花粉漂移距离和强度进行了观测,并应用PCR法检测转Bt基因棉的基因流频率.花粉粒染色法监测结果表明:同株异花间的花粉散布频率显著高于异株异花间(P<0.01);靠近转Bt基因棉花粉染色区1m处的平均花粉散布频率,在东、南、西、北4个方向分别为44.8%、48.9%、57.1%和21.5%,但随着距转基因棉田距离的增大,4个方向的平均花粉散布频率都呈下降趋势.PCR结果的统计分析表明,在25m内,花粉散布距离和方向对基因流频率有极显著影响(P<0.01),随着距转基因棉田距离的增大,基因流频率呈下降趋势,最远距离为25m时的最高基因流频率为2.0%.
译  名：
Monitoring of pollen-mediated gene flow from transgenic Bt cotton
作  者：
WANG Chang-yong1,2, LIU Yan2, ZHOU Jun1, CHEN Jian-qun1, QIN Pei1 (1School of Life Sciences, Nanjing University, Nanjing 210093, China; 2Nanjing Institute of Environmental Sciences, State Environmental Protection Administration of China, Nanjing 210042, China).
关键词：
transgenic Bt cotton; gene flow; pollen dispersal; pollen grain dyeing; monitoring
摘  要：
By the methods of pollen grain dyeing and PCR analysis, this paper measured the distance and intensity of pollen dispersal, and determined the frequency of gene flow (FGF) of transgenic Bt cotton. The results showed that the frequency of pollen dispersal (FPD) among flowers was significantly higher on the same cotton plant than on different ones (P<0.01). The average FPD at a distance of 1 m from the dyed Bt cotton plot to its east, south, west and north was estimated to be 44.8%, 48.9%, 57.1% and 21.5%, respectively, and declined with increasing distance in the four directions. Within the range of 25 m, the distance and direction of pollen dispersal could affect FGF significantly (P<0.01), and FGF generally tended to decrease with increasing distance from the Bt cotton plot, with a frequency of 2.0% at the farthest distance of 25 m.

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