PCR detection of TRIMCyp genotype in Macaques from hair follicle DNA

TANG Yan-dong1,YU Chang-qing1,LIU Xiao-ming2,CUI Duo-ying3,NA Lei1,4,RAO Jun-hua2,JI Fang2,LV Xiao-ling1,ZHENG Yong-hui5,ZHOU Jian-hua1(1.State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China;2.South-China Primate Research and Development Center,Guangdong Entomological Institute,Guangzhou 510260,China;3.Beijing Zoo,Beijing 100044,China;4.College of Life Science,Northeast Agricultural University,Harbin 150030,China;5.Microbiology and Molecular Genetics,Michigan State University,MI48824,USA)

hair follicle PCR;Macaques;TRIM5-alpha;genotype;HIV-1

A PCR-based method was developed to detect the TRIMCyp genotypes in macaques from genomic DNA extracted from hair follicle.This method could specifically amplify PCR products of 2 343 bp for TRIMCyp homozygote,2 343 bp and 3 110 bp for heterozygote from genomic DNA of Macaca fascicularis,Macaca nemestrina and Macaca mulatta.It was highly reproducible regardless of genomic DNA extracted from hair follicle or from blood leukocyte cells.The application of hair follicle genomic PCR was a sensitive and easy-to-use approach for investigation of the distribution of TRIMCyp in different species of genus Macaca.Monkeys with unique genotype was important models for studies on cross-species infection of retroviruses and on the development of novel nonhuman primate models for HIV-1.