作 者：
高林;廖德芳;姚俊;张念祖;李华春;李志华
单 位：
云南省畜牧兽医科学院云南省热带亚热带动物病毒病重点实验室农业部热带亚热带动物病毒学重点开放实验室
关键词：
乙基环丙沙星;大肠杆菌;BHK-21细胞;清除污染
摘 要：
为重建受大肠杆菌污染的BHK-21细胞,本研究采用药物乙基环丙沙星,先测出该药对大肠杆菌的最低抑制浓度(minimum inhibitory concentration,MIC),再测出该药对BHK-21细胞的MIC,从而确定10mg/L为乙基环丙沙星不影响细胞生长,又能抑制大肠杆菌的安全浓度。然后用含该浓度乙基环丙沙星的MEM生长液,对受污染的BHK-21细胞进行物理、化学相结合的循环处理。经过3次循环处理,再传3代后,确认大肠杆菌污染已经被彻底清除,细胞恢复了健康。此方法为抢救受细菌污染的细胞提供了参考。
译 名：
Reconstruct the Contaminated BHK-21 Cells by Escherichia coli with Enrofloxacin
作 者：
GAO Lin,LIAO De-fang,YAO Jun,ZHANG Nian-zu,LI Hua-chun,LI Zhi-hua(Key Laboratory of Tropical & Subtropical Animal Virology of the Ministry of Agriculture, Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory,Yunnan Animal Science and Veterinary Institute,Kunming 650224,China)
关键词：
Enrofloxacin;Escherichia coli;BHK-21 cells;clean contamination
摘 要：
The aim is to reconstruct the BHK-21 cells contaminoted by Escherichia coli using Enrofloxacin.This study tested the MIC of Escherichia coli and BHK-21 cells,found the safe concentration of Enrofloxacin was 10 mg/L,it didn't effect the growth of BHK-21 cells,and could inhibit the Escherichia coli.By physical and chemical method,the contaminated BHK-21 cells were treated with the MEM growing solution of the same concentration of Enrofloxacin circularly.The Escherichia coli were cleaned successfully after 3 cycles and 3 passages,the BHK-21 cells were recovered.The method offered a reference for saving the contaminated cells.
