当前位置: 首页 > 文章 > 利用ISSR标记分析青麻种质资源遗传多样性 中国农业科学 2008,41 (11) 3532-3541
Position: Home > Articles > Genetic Diversity of Velvetleaf Germplasm Revealed by ISSR Marker Scientia Agricultura Sinica 2008,41 (11) 3532-3541

利用ISSR标记分析青麻种质资源遗传多样性

作  者:
白占兵;粟建光;陈基权;戴志刚;王凤敏;龚友才
单  位:
中国农业科学院麻类研究所
关键词:
青麻;种质资源;ISSR;遗传多样性;起源
摘  要:
【目的】利用分子标记分析来自中国11个省(市)的48份青麻种质资源的遗传多样性,为青麻资源利用和育种提供分子生物学依据。【方法】利用内部简单重复序列多态性(ISSR)分子标记检测青麻种质资源的遗传多样性。【结果】在48份青麻种质资源中,9条ISSR引物扩增出82条带,多态条带比率(PPB)为88.89%,扩增产物片段大小在0.1~3.0kb。基于UPGMA聚类,将青麻分为3大类。【结论】中国青麻的遗传多样性水平较高,实验结果支持青麻起源于中国。
译  名:
Genetic Diversity of Velvetleaf Germplasm Revealed by ISSR Marker
作  者:
BAI Zhan-bing, SU Jian-guang, CHEN Ji-quan, DAI Zhi-gang, WANG Feng-min, GONG You-cai (Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205)
关键词:
Velvetleaf; Germplasm; ISSR; Genetic diversity; Origin
摘  要:
【Objective】This article analyzed the genetic diversity of 48 velvetleaf from 11 provinces of China, and provided biological information on utilization and breeding of velvetleaf germplasm.【Method】Inter-simple Sequence Repeat (ISSR) markers were applied to detect genetic diversity of 48 velvetleaf germplasm in China. 【Result】82 bands were amplified with nine ISSR primers, with 88.89% polymorphic ones. The amplified DNA fragments were 0.1-3.0 kb in size. The dendrogram was constructed by UPGMA method, showing 48 velvetleaf germplasm clustered into three groups. 【Conclusion】There is a high genetic diversity in velvetleaf germplasm. The result supported the conclusion that China is the origin of velvetleaf.

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