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当前位置: 首页 > 文章 > 枣瘿蚊微卫星标记筛选及微卫星多态性分析 新疆农业科学 2020 (5) 888-894
Position: Home > Articles > Microsatellite Markers Dressing by Screening and Polymorphism Analysis of Contarinia datifolia Xinjiang Agricultural Sciences 2020 (5) 888-894

枣瘿蚊微卫星标记筛选及微卫星多态性分析

作  者：
马光皇;康淑媛;李莉;肖海兵;熊仁次;杨明禄
单  位：
塔里木大学植物科学学院
关键词：
枣瘿蚊;微卫星(SSR);多态性;遗传多样性
摘  要：
【目的】研究枣瘿蚊基因遗传变异程度,分析基因多态性。【方法】从枣瘿蚊部分基因组序列中搜索SSR并设计相应引物,挑选引物48对进行有效筛选。枣瘿蚊样本来自新疆阿拉尔市、昆玉市、且末县等地区,以枣瘿蚊DNA为模板进行PCR扩增,筛选特异性微卫星引物;通过琼脂糖凝胶电泳进行定性检测,再以毛细管电泳检测其多态性。【结果】有42对引物能扩增出条带,选择其中20对特异性较好的引物进行多态性分析。观测杂合度(Observed heterozygosity,Ho)、期望杂合度(Expected heterozygosity,He)和多态信息含量(PIC)平均值分别是0.358、0.649和0.635,其中18对引物PIC值>0.5,具有较高的多态性。【结论】开发的微卫星标记多态性较高,可用于枣瘿蚊种群遗传多样性及遗传结构研究。
译  名：
Microsatellite Markers Dressing by Screening and Polymorphism Analysis of Contarinia datifolia
作  者：
MA Guanghuang;KANG Shuyuan;LI Li;XIAO Haibing;XIONG Renci;YANG Minglu;College of Plant Science, Tarim University;Southern Xinjiang Key Laboratory of IPM of Tarim University;Scientific Observing and Experimental Station of Crop Pests in Aral, Ministry of Agriculture,P.R China;
关键词：
Dasineura jujubifolia;;microsatellties;;polymorphism;;genetic diversity
摘  要：
【Objective】 The goal was to study the genetic variability of Dasineura jujubifolia genes and analyze its genetic polymorphisms.【Method】 In this experiment, SSR was searched from genome sequences of Dasineura jujubifolia and corresponding primers were designed, from which 48 pairs of primers were selected for effective screening. The Dasineura jujubifolia samples came from Aral, Kunyu and Qiemo and they were amplified by PCR using Dasineura jujubifolia DNA as template. Qualitative detection was performed by agarose gel electrophoresis, and the polymorphism was detected by capillary electrophoresis.【Result】The results show that:There were 42 pairs of primers that could amplify the bands, and 20 with good specificity of them were selected for polymorphism analysis. The average values of observed heterozygosity(Ho), expected heterozygosity(He) and polymorphic information content(PIC)were 0.358, 0.694 and 0.635. Among them, the PIC of 18 pairs of primers was more than 0.5, which was highly polymorphic.【Conclusion】SSR that we developed have high polymorphisms, which can be used to study the genetic diversity and genetic structure of Dasineura jujubifolia population.

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