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Position: Home > Articles > Research on the number of mucosal immunity-associated cell in porcine proliferative enteritis Journal of Northeast Agricultural University 2011,42 (12) 25-29+146

猪增生性肠炎黏膜免疫相关细胞数量研究

作  者:
陈彤;杨小燕;祁保民
单  位:
福建农林大学动物科学学院;龙岩学院生命科学学院;福建省人畜寄生与病毒性疫病防控工程技术研究中心
关键词:
猪;猪增生性肠炎;小肠;淋巴细胞;杯状细胞;肥大细胞
摘  要:
为了揭示猪增生性肠炎黏膜免疫相关细胞数量的变化情况。采用组织学方法,比较PCR检验阳性猪(获得胞内劳森氏菌特异性扩增产物)与阴性猪(无胞内劳森氏菌特异性扩增产物)的十二指肠、空肠、回肠黏膜淋巴细胞、杯状细胞、肥大细胞的数量和分布变化。结果表明,阳性猪十二指肠、空肠黏膜上皮内每100个柱状细胞间淋巴细胞的数量同比阴性猪稍微下降(P>0.05),回肠同比有所升高(P>0.05);阳性猪十二指肠、空肠和回肠黏膜上皮内每100个柱状细胞间杯状细胞的数量同比阴性均显著增多(P<0.05);阳性猪空肠、回肠黏膜固有层内肥大细胞的数量同比阴性猪均显著增多(P<0.05)。该研究有助于了解猪增生性肠炎肠道黏膜免疫屏障功能的状态,为该病的防治提供参考。
译  名:
Research on the number of mucosal immunity-associated cell in porcine proliferative enteritis
作  者:
CHEN Tong1,2,YANG Xiaoyan1,2,QI Baomin3(1.College of Life Sciences,Longyan University,Longyan Fujian 364000,China;2.Engineering Research Center for the Prevention and Control of Zoonosis,Longyan Fujian 364000,China;3.College of Animal Science,Fujian Agricuture and Fonestry University,Fuzhou,350002,China)
关键词:
pig;porcine proliferative enteritis;small intestine;lymphocyte;goblet cell;mast cell
摘  要:
In order to reveal change of the number of mucosal immunity-associated cell in porcine proliferative enteritis.Using light microscope method to compare the number and distribution change of duodenum,jejunum,ileum mucous membrane lymphocyte,goblet cell,and mast cell of the positive swine(with Lawsonia intracellularis specificity amplified products) and the negative swine(without Lawsonia intracellularis specificity amplified products)after the PCR test.The results showed that: In duodenum and jejunum mucous membrane epithelium cell of the positive swine,compared with the negative swine,lymphocyte's number in each 100 columnar cells decreased slightly(P>0.05),ileum the same increased slightly(P>0.05);In duodenum,jejunum and ileum mucous membrane epithelium cell of the positive swine,compared with the negative swine,goblet cell's number in each 100 columnar cells increased significantly(P<0.05);Mast cell's number in mucosal lamina propria of the jejunum and ileum in positive swine,compared with the negative swine,increased significantly(P<0.05).The research helps to understand the status of intestinal mucosa′s immunological barrier function in porcine proliferative enteritis and provides a reference for the prevention and treatment of the disease.

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