中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 取食转Bt基因水稻对稻纵卷叶螟幼虫中肠的组织病理学效应 昆虫学报 2007,50 (10) 1070-1076
Position: Home > Articles > Histopathological changes in the midgut of the rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) larvae feeding on transgenic Bt rice Acta Entomologica Sinica 2007,50 (10) 1070-1076

取食转Bt基因水稻对稻纵卷叶螟幼虫中肠的组织病理学效应

作  者：
李芳芳;叶恭银;吴琼;彭予发;陈学新
单  位：
浙江大学昆虫科学研究所水稻生物学国家重点实验室;中国农业科学院植物保护研究所
关键词：
稻纵卷叶螟;转Bt基因水稻;中肠;组织病理变化;透射电镜
摘  要：
利用透射电镜观察了稻纵卷叶螟Cnaphalocrocis medinalis(Guene)幼虫取食转Bt基因水稻后中肠的组织病理变化。结果表明:稻纵卷叶螟幼虫取食转cry1Ab基因水稻后,中肠上皮细胞的线粒体先发生形态变化,随连续取食时间的延长线粒体出现凝聚、内嵴稀疏、空泡化等,在后期还呈凝聚态随突起脱落或沿杯腔边沿单一排列。内质网的变化也很明显,病变过程中伴随着粗糙内质网的肿胀、核糖体脱落,粗糙内质网增多等现象。细胞核的变化较小,在处理后期出现细胞核拉长、核仁聚集等变化。组织病变程度不一,有的细胞在病变早期就出现了空泡化。
译  名：
Histopathological changes in the midgut of the rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) larvae feeding on transgenic Bt rice
作  者：
LI Fang-Fang1, YE Gong-Yin1, WU Qiong1, PENG Yu-Fa2, CHEN Xue-Xin 1, (1. State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China; 2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China)
关键词：
Cnaphalocrocis medinalis; transgenic Bt rice; midgut; histopathological change; transmission electron microscopy
摘  要：
The histopathological changes of the midgut of rice leaf folder, Cnaphalocrocis medinalis (Guenée) larvae feeding on Bt rice line "KMD1" and "KMD2" were observed using transmission electron microscopy. The results showed that cellular alternations in the midgut tissues, such as swelling of mitochondria, endoplasmic reticulum, and dilatation of intercellular spaces, fenestration or disorganization of the Golgi complex, and concentric arrangement of rough endoplasmic reticulum. The initial infected organelles were mitochondria. After longer feeding on Bt rice by the larvae, the upper portions of the cells swelled, and cytoplasm in these regions decreased in electronic density and other organelles were damaged to a certain degree, such as wiredraw of nucleus, condensation of nuclear, and the increase of number of rough endoplasmic reticulum. As the infection proceeded further, pathological changes in the mitochondrial abundant areas of basal membrane enfoldings of columnar cells and cytoplasmic projections of goblet cells became more obvious. Finally, the midgut epithelium cells disrupted and sloughed off from the basal membrane.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46