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当前位置: 首页 > 文章 > 基于元分析的大豆含硫氨基酸相关基因挖掘与信息学分析 中国油料作物学报 2015,37 (2) 141-147
Position: Home > Articles > Mining and analysis of genes related to sulfur- containing amino acids in soybean based on Meta- QTL Chinese Journal of Oil Crop Sciences 2015,37 (2) 141-147

基于元分析的大豆含硫氨基酸相关基因挖掘与信息学分析

作  者：
邱红梅;厉志;于妍;高淑芹;马晓萍;郑宇宏;孟凡凡;侯云龙;王跃强
单  位：
长春科技学院;东北农业大学大豆生物学教育部重点实验室;吉林省农业科学院大豆研究所/大豆国家工程研究中心
关键词：
大豆;含硫氨基酸;元分析;Meta-QTL;候选基因
摘  要：
提高含硫氨基酸组分是大豆品质改良的重要目标之一。本文借助Consensus Map 4.0高密度大豆遗传图谱,整合了33个含硫氨基酸QTL,并采用元分析方法,确定了8个含硫氨基酸Meta-QTL。通过QTL序列共线性区间分析,在Meta-QTL内筛选到7条用于候选基因发掘的基因组序列。利用基因功能注释,得到16个含硫氨基酸相关基因,包括6个氨基酸合成途径酶基因、1个11S大豆球蛋白A5A4B3亚基基因及9个调控基因。其中调控基因分别编码F-Box、PPR及转录因子3种蛋白。13个基因在NCBI数据库中分别有表达谱及SNP信息。大豆含硫氨基酸候选基因分析,可以为功能标记的开发和分子辅助育种提供有用信息。
译  名：
Mining and analysis of genes related to sulfur- containing amino acids in soybean based on Meta- QTL
作  者：
QIU Hong-mei;LI Zhi;YU Yan;GAO Shu-qin;MA Xiao-ping;ZHENG Yu-hong;MENG Fan-fan;HOU Yun-long;WANG Yue-qiang;WANG Shu-ming;Key Laboratory of Soybean Biology in Chinese Ministry of Education,Northeast Agricultural University;Soybean Research Institute,Jilin Academy of Agricultural Sciences /National Engineering Research Center for Soybean;Changchun University of Science and Technology;
关键词：
Soybean;;Sulfur-containing amino acids;;Meta-analysis;;Meta-QTLs;;Candidate genes
摘  要：
Increasing the sulfur- containing amino acid content has been one of the most important goals to improve soybean quality. The objectives of this paper were to discover the soybean sulfur- containing amino acid Meta- QTLs and candidate genes related to sulfur- containing amino acid components in order to establish a basis of developing functional markers for the sulfur- containing amino acids. 33 QTLs related to sulfur- containing amino acids in soybean were integrated by using the reference map of Consensus Map 4. 0 high density soybean genetic map. By Meta- analysis method,8 Meta- QTLs for sulfur- containing amino acids were estimated. 7 long sequences used for screening candidate genes were identified in Meta – QTLs,by collinear interval of QTL sequence. 16 sulfur- containing amino acid related genes were acquired by using function annotation of genes,including 6 genes of amino acid synthetic enzyme,1 gene of 11 s glycinin A5A4B3 and 9 regulated genes. Regulated genes respectively encoded F- Box protein,PPR protein and transcription factors. There were EST profiles and SNPs of 13 genes in NCBI database. Analysis of candidate genes related to sulfur- containing amino acids in soybean could provide useful information for molecular marker assisted breeding.

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