当前位置: 首页 > 文章 > 山羊核移植胚胎干细胞的分离培养 安徽农业科学 2011,39 (7) 4012-4014
Position: Home > Articles > Isolated Culture of Nuclear Transplantation Embryonic Stem Cells of Goat Journal of Anhui Agricultural Sciences 2011,39 (7) 4012-4014

山羊核移植胚胎干细胞的分离培养

作  者:
葛秀国;李吉霞;窦忠英
单  位:
天津农学院动物科学系;西北农林科技大学陕西省干细胞工程技术研究中心
关键词:
山羊;核移植;胚胎干细胞
摘  要:
比较了不同发育阶段山羊核移植胚胎分离培养胚胎干细胞(NT-ESCs)的情况。结果表明,各阶段山羊核移植胚胎均可分离出NT-ESCs,其中孵化胚的NT-ESCs克隆形成率最高(38%),与囊胚(26%)差异显著,与桑葚胚(18%)差异极显著。所分离的NT-ESCs细胞形态与正常ESCs相似,均具有碱性磷酸酶活性,可表达多能性基因Oct-4和表面标志抗原SSEA-1。
译  名:
Isolated Culture of Nuclear Transplantation Embryonic Stem Cells of Goat
作  者:
GE Xiu-guo et al(Department of Animal Science,Tianjin College of Agronomy,Tianjin 300384)
关键词:
Goat;Nuclear transplantation;Embryonic stem cells
摘  要:
The isolated cultures of nuclear transplantation embryonic stem cells(NT-ESCs) from nuclear transplantation embryos of goats in different developmental stages were compared.The results showed that NT-ESCs could be isolated from nuclear transplantation embryos of goats in all developmental stages,among which the cloning efficiency of NT-ESCs from hatching embryos was highest,being 38%;it had significant difference from that of blastula,which was 26% and it had extremely significant difference from that of embryotic sphere,which was 18%.The shape of isolated NT-ESCs was similar to that of normal ESCs,both of them had alkaline phosphatase activities and could express pluripotency gene Oct-4 and surface marker antigen SSEA-1.

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