作 者：
姜锦鹏;韦习会;陈杰;陆天水;池豪;赵茹茜
单 位：
南京农业大学动物医学院/农业部动物生理生化重点开放实验室;上海华扩达生化技术研究开发有限公司
关键词：
猪;抗体;脂肪细胞膜;胴体品质;脂蛋白脂酶活性
摘 要：
【目的】研究口服型脂肪细胞膜蛋白抗体对生长肥育猪胴体品质和代谢的影响及其作用机理。【方法】体重27kg左右的杜×长·梅三元杂交商品猪160头,随机分成试验组和对照组。试验组基础日粮中添加75mg·kg-1脂肪细胞膜蛋白抗体,对照组在基础日粮中添加等量阴性抗体辅料,饲喂104d后屠宰,采集血液和组织样品。用放射免疫法测定血清胰岛素(insulin)和瘦素(leptin)浓度,组织学方法测量脂肪细胞直径,分光光度法测定脂肪组织DNA、RNA浓度、脂肪组织氧化型辅酶Ⅱ-苹果酸脱氢酶(NADP-MDH)活性以及脂肪与肌肉组织中脂蛋白脂酶(LPL)活性。【结果】试验组平均日增重及瘦肉率分别提高13.03%(P<0.01)和10.30%(P<0.01),背膘厚以及肾周脂肪、肠系膜脂肪和皮下脂肪比率分别下降24.14%(P<0.01)、27.27%(P<0.05)、20.42%(P<0.01)和29.21%(P<0.01),而肌内脂肪含量则不受影响;试验组血清insulin浓度降低26.19%(P<0.05),leptin浓度下降26.53%(P<0.05);不同部位脂肪细胞的直径显著降低;脂肪组织NADP-MDH活性变化不显著;脂肪组织脂蛋白脂酶活性以及脂肪组织与肌肉组织脂蛋白脂酶活性的比值显著下降(P<0.05)。【结论】口服脂肪细胞膜蛋白抗体可显著改善猪的胴体品质;血清insulin、leptin以及组织脂蛋白脂酶活性的改变可能参与这种作用的调节。
译 名：
Effect of Adipocyte Plasma Membrane Antibody on Carcass Composition and Lipid Metabolism-Related Hormones and Enzymes in the Pig
作 者：
JIANG Jin-peng1,3,WEI Xi-hui1,CHEN Jie1,LU Tian-shui1,CHI Hao2,ZHAO Ru-qian1 (1Key Laboratory of Animal Physiology and Biochemistry/College of Veterinary Medicine,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095; 2Walcom Bio-chemicals Industrial Limited,Shanghai 201206; 3Anhui Science and Technology University,Bengbu 233100)
关键词：
Pigs; Antibody; Adipocyte plasma membrane; Carcass composition; LPL activity
摘 要：
Objective The study looked at the effects of oral type antibody against adipocyte plasma membrane (OAAb) on the metabolism,carcass composition and its mechanisms in pigs.MethodCrossbred (Duroc-Jersey× Landrace·Meishan) pigs(160 of them) weighing approximately 27 kg were allocated randomly into treatment and control groups. The treatment group was fed with the basal diet supplemented with 75 mg·kg-1 OAAb. Following a 104-day trial,the pigs were slaughtered,the carcass composition was measured and the weights of internal fat depots were recorded. Blood and tissue samples were taken for further analysis. Serum concentrations of insulin and leptin were determined with RIA. Diameters of adipocytes from different fat depots were measured by using histological methods. DNA/RNA concentrations and NADP-MDH,lipoprotein lipase (LPL) activity were analyzed. Result Dietary supplementation of OAAb increased average daily weight gain and lean mass by 13.03% (P<0.01) and 10.3% (P<0.01),respectively,and dressing percentage was unaffected. Back fat thickness at 6th-7th rib was reduced by 24.14% (P<0.01),the perirenal,mesenteric and subcutaneous fat depots were reduced by 27.27% (P<0.05),20.42% (P<0.01) and 29.21% (P<0.01),however,the intramuscular fat content of the longissimus muscle remained unaffected. Serum concentrations of insulin and leptin were reduced by 26.19% (P<0.05) and 26.53% (P<0.05),respectively. Dietary supplementation of OAAb reduced adipocyte size in all the three different adipose pads (P<0.05),LPL activity in adipose tissue and the ratio of LPL activity between adipose tissue and skeletal muscle were reduced significantly (P<0.05) whereas NADP-MDH activity was unaffected. Conclusion We first demonstrated the oral effect of OAAb on carcass composition in the pig and the changes of serum insulin and leptin levels as well as the tissue LPL activity may be involved in the acting mechanism.
