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当前位置: 首页 > 文章 > 甜瓜幼苗不同叶位SPAD值与叶绿素含量的变化规律及相关性 被引量:1 华北农学报 2019 (S01) 99-104
Position: Home > Articles > Variation and Correlation Analysis of SPAD Value and Chlorophyll Content of Leaves at Different Position in Melon Seedling Acta Agriculturae Boreali-Sinica 2019 (S01) 99-104

甜瓜幼苗不同叶位SPAD值与叶绿素含量的变化规律及相关性 被引量:1

作  者：
宋慧;黄芸萍;臧全宇;张凌霄;张香琴
单  位：
宁波市农业科学研究院
关键词：
甜瓜;叶绿素;SPAD;测量叶
摘  要：
甜瓜两叶一心期是幼苗植株对低温弱光敏感时期.明确该时期甜瓜不同叶位SPAD值与叶绿素含量的变化规律与相关性,可以为SPAD值无损预测甜瓜叶绿素含量、鉴定甜瓜耐低温弱光特性科学选择测量叶.以甜瓜自交系P148两叶一心期幼苗为试材,测量自下而上3片新叶(Y1、Y2和Y3)SPAD值和叶绿素含量,利用SPSS 13.0软件One-Way-ANOVA程序和Correlation程序,分析甜瓜两叶一心期3片新叶叶绿素a、b和a+b含量的变化规律和与SPAD值的相关性;最后使用Regression程序,选择SPAD值与叶绿素含量相关系数最大的叶位,建立SPAD值与叶绿素含量回归方程,以此叶位作为甜瓜两叶一心时期利用SPAD值预测叶绿素含量的测量叶.结果表明,甜瓜两叶一心期叶片SPAD值和叶绿素含量随着叶位升高而增加,Y3的叶绿素含量极显著地高于Y1和Y2;3个叶位叶绿素含量与SPAD的相关性随着叶位升高而降低,Y1的Chla+b含量与SPAD值的相关性最高,建立回归方程:y(Chla+b)=0.032x(SPAD)-0.141,决定系数(R2)=0.699;Y3的叶绿素含量与SPAD值无显著相关性.最终选择该时期第一片新叶作为利用SPAD值无损预测甜瓜叶绿素含量的测量叶,为批量筛选甜瓜耐低温弱光材料提供可操作方案.
译  名：
Variation and Correlation Analysis of SPAD Value and Chlorophyll Content of Leaves at Different Position in Melon Seedling
摘  要：
Two leaves and one new period was the sensitive period of melon to low temperature and low light condition,which period was always used to chlorophyll content determination and tolerance material selection.There were three leaves in this period,but no research mentioned about the definite measure leaf.To understand these and select the suitable measure leaf for chlorophyll content prediction by SPAD value determination,the chlorophyll content and SPAD value of three leaves of two leaves and one new period melon were collected and analyzed.The three leaves from bottom to up(Y1,Y2 and Y3)at different position of melon inbred line of P148 was selected to determinate and analyze the variation and correlation of SPAD value and chlorophyll content in two leaves and one new period by One-Way-ANOVA,Correlation and Regression program of SPSS 13.0 soft,respectively.The SPAD value and chlorophyll content were increased with increasing of leaves position.The Y3 showed the higher SPAD value and chlorophyll content than Y1 and Y2 significantly.The correlation between SPAD value and chlorophyll content was reduced with increasing of leaves position.The SPAD value and chlorophyll(a+b)content of Y1 had highest R value,the regression equation was established:y(Chla+b)=0.032x(SPAD)-0.141,R2=0.699.The first leaf was selected to determinate the SPAD value for chlorophyll content prediction to mass screen the low-temperature-and-light tolerance melon.

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