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Position: Home > Articles > THE USE OF RADIOIMMUNO-PEG ASSAY FOR THE DETECTION OF CIRCULATING IMMUNO-COMPLEXES AND ANTIBODY OF ACUTE TOXOPLASMOSIS IN SWINE Chinese Journal of Animal and Veterinary Sciences 1984,15 (2) 1-5

放射免疫法检测急性弓形体病猪血清中循环免疫复合物和抗体

作  者:
郑庆端;计浩;周元根;吴美珍;马以武;章胜林;王菊芳
摘  要:
本文用放射免疫法(Radioimmunoassay,RIA)检测急性弓形体病猪血清110份,经感染后第五天血清中出现明显的弓形体特异性抗体和免疫复合物,而健康对照猪血清10份全部阴性。此种抗体与免疫复合物连续测定26天,其结果一直保持在阳性范围之内。急性弓形体病的临床期诊断尤其对人的弓形体病更为重要。目前国内外都采用血清学方法检测康复后血清抗体,但是此法未能做出快速确诊,从而拖延时间影响了对症治疗。放射免疫法以抗原和抗体特异性免疫反应为基础,是一种超微量分析技术,它能够探测101~(-12)克水平元素,其方法具有灵敏、精密度高,特异性强等的优点。上述研究将为今后建立快速诊断弓形体病提供新的测试手段,值得推广使用。
译  名:
THE USE OF RADIOIMMUNO-PEG ASSAY FOR THE DETECTION OF CIRCULATING IMMUNO-COMPLEXES AND ANTIBODY OF ACUTE TOXOPLASMOSIS IN SWINE
作  者:
CHENG.C.T JI.H CHOU Y.G WU.M.C MA.J.W CHONG.S.L WANG.Z,F (Institute of Animal Husbandr y & Veterinary Science,Jiangsu Academy of Agricultural Sciences;People's Hospital of Quan-Shan District,Jiangsu.)
摘  要:
By the use of radioimmuno-precipitation assay for the.detection of circulating immuno-complexes and antibody of acute toxoplasmosis with 110 sampes of serum of infected pigs,it was found that the serum con- tained specific antibody and circulating immuno-compicxes 5 days post- infection while it was negative in the 10 control serum samples.These circulating materials could be detected as long as 26 days with apeakat 9th day and their persitence in the positive limit were quite stable. Since this method was specific,reliable,very sensitive,and easy to perform,it was considered to be a valuable tool as rapid diagnosis for swine Toxopasmosis.

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