当前位置: 首页 > 文章 > 胡子鲇肥大细胞的组织化学及形态学 中国水产科学 2003,10 (2) 106-110
Position: Home > Articles > Hishtochemistry and morphology of mast cells in cat fish Claris fuscus lacepede Journal of Fishery Sciences of China 2003,10 (2) 106-110

胡子鲇肥大细胞的组织化学及形态学

作  者:
杨筱珍;高登慧;许乐仁
单  位:
贵州大学生物技术学院动物医学系
关键词:
肥大细胞;组织化学;形态学;胡子鲇
摘  要:
以成年胡子鲇(Clarisfuscuslacepede,体重1.5kg)为研究材料,通过组织化学与形态学研究证实,肥大细胞在胡子鲇肠粘膜与粘膜下数量较多,在头肾和胸腺中也有少量分布,并有沿血管周分布的倾向。肥大细胞以Carnoy氏固定、AB/SO染色的效果最好。以AB/SO着色的肥大细胞胞质颗粒呈深蓝色,胞核呈淡褐色;以TB/SO着色的肥大细胞胞质颗粒呈紫红色,胞核呈淡红色。而中性缓冲福尔马林固定对肥大细胞的着染有一定阻断作用。一些曾被国外学者用于鉴定硬骨鱼肥大细胞(或嗜酸性颗粒细胞)的组织化学技术在本研究中未能鉴定出胡子鲇的肥大细胞。电镜观察证实,胡子鲇肥大细胞中含有大量特征性的胞浆颗粒,胞浆颗粒基质电子密度较均匀,个别颗粒中央呈空泡样。
译  名:
Hishtochemistry and morphology of mast cells in cat fish Claris fuscus lacepede
作  者:
YANG Xiaozhen, GAO Denghui,XU Leren(Department of Animal Medicine, College of Biotechnology, Guizhou University, Guiyang 550025, China)
关键词:
mast cell; histochemistry; morphology; Claris fuscus lacepede
摘  要:
Mast cells were firstly identified in the tissues of cat fish (Claris fuscus lacepede) histochemically and morphologically. Numerous mast cells can be found in the mucosa and submucosa of the intestine and a few in the thymus and the head kidney of cat fish, and the mast cell intends to adjacent to blood vessels. It seems that the staining properties of the mast cells in cat fish are similar to the mucosal mast cells in mammals in some aspects. Carnoy's fluid and Alcian blue are good fixative and excellent dye for the mast cells, but fixation in neutral buffered formalin (NBF) was unsatisfactory. Some other histochemical methods previously used by researchers to detect mast cells (EGC) in teleostean fish are failed to identify the mast cells in cat fish in this study. Under electron microscopy, the mast cells in cat fish contain characteristic cytogranules and the electron density of the matrix in the granules is homogerous, but in a few granules a holelike substructure can be seen occasionally.

相似文章

计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊