Proteomic analysis of pathogen-responsive proteins from the bark of apple branches induced by Botryosphaeria dothidea

ZHANG Cai-xia;TIAN Yi;ZHANG Li-yi;XIAO Long;KANG Guo-dong;CONG Pei-hua;Key Laboratory of Horticulture Crops Germplasm Resources Utilization,Ministry of Agriculture;Research Institute of Pomology,Chinese Academy of Agricultural Sciences;

bark of apple branches;;total protein;;Botryosphaeria dothidea;;stress;;differential proteomics;;mass spectrometry

The optimal protein extraction method is the key for proteomics research by tw o-dimensional electrophoresis( 2-DE). In this study,in order to establish a suitable method for extraction of total protein from the bark of apple branches,the extraction condition for the 2-DE w as optimized. To elucidate the key proteins related to disease-resistance of apple branches induced by Botryosphaeria dothidea,total proteins w ere extracted from control and infected branches, and separated by tw o-dimensional electrophoresis. The differentially expressed proteins w ere then identified by M ALDI-TOF-TOF / M S. In total,25 differentially expressed proteins w ere detected by Image M aster Softw are. After tryptic digestion,M ALDI-TOF-TOF / M S analysis and database searching,22 protein spots w ere finally identified. The predicted function of 22 proteins w ere related to photosynthesis,glucose / energy metabolism,defense responses,protein synthesis and other unknow n functions.The pathogenesis-related proteins( APX,β-1,3-glucanase,M al d1 and PR-1) involved in defense responses and differentially expressed in apple branches may play a key role in resistance to B. dothidea.