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Position: Home > Articles > Development of A Polymerase Chain Reaction Assay for Detection of Haplosporidium in Shellfish China Animal Husbandry & Veterinary Medicine 2010,37 (1) 57-59

贝类单孢子虫PCR检测方法的建立

作  者:
谢丽基;谢芝勋;庞耀珊;刘加波;邓显文;谢志勤
单  位:
广西兽医研究所
关键词:
贝类;单孢子虫;PCR检测方法
摘  要:
根据基因库中单孢子虫的基因保守序列,设计了1对特异性引物,通过对PCR扩增条件的优化,建立了检测贝类单孢子虫的PCR方法。用该方法对单孢子虫模板进行扩增,得到与试验设计相符的244 bp的特异性条带,而对派琴虫、折光马尔太虫、嗜水气单胞菌、荧光假单胞菌、副溶血弧菌、溶藻弧菌、河弧菌和拟态弧菌等病原体的扩增结果全为阴性。敏感性试验结果表明,该方法最低能检测到100 fg的单孢子虫DNA。
译  名:
Development of A Polymerase Chain Reaction Assay for Detection of Haplosporidium in Shellfish
作  者:
XIE Li-ji,XIE Zhi-xun,PANG Yao-shan,LIU Jia-bo,DENG Xian-wen,XIE Zhi-qin(Guangxi Veterinary Research Institute,Nanning 530001,China)
关键词:
Shellfish;Haplosporidium sp;PCR
摘  要:
According to the gene sequences in GenBank of Haplosporidium sp,a pair of specific primers were designed for amplifying the specific fragments of Haplosporidium sp.The reaction parameters were optimized to develop the polymerase chain reaction method for detection of Haplosporidium sp.The 244 bp long specific DNA fragment of Haplosporidium sp was amplified,but not from other pathogenic such as Perkinsus sp,Marteilia refringens,Aeromonas hydrophila,Pseudomonas fluorescens,Vibrio parahaemolyticu,Vibrio Alginolyticu,Vibrio Fluvialis and Vibrio Mimicus by PCR.The sensitivity results showed that as little as 100 fg DNA of Haplosporidium sp was detected by this PCR.

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