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Position: Home > Articles > Change of MBL in serum and histopathologic analysis after artificially infecting Mycoplasma ovipneumoniae Chinese Journal of Veterinary Medicine 2015,51 (2) 9-12+49

人工感染绵羊肺炎支原体后绵羊甘露聚糖结合凝集素变化及组织病理学

作  者:
王小义;张红梅;古丽汗·阿不都热木;郭翠翠;赵宗胜
单  位:
石河子大学动物科技学院;石河子大学生命科学学院;山东省青州第一中学;石河子大学附属医院
关键词:
绵羊;甘露聚糖结合凝集素;组织病理学评分;绵羊肺炎支原体
摘  要:
为探讨绵羊甘露聚糖结合凝集素(Mannose-binding lectin,MBL)对绵羊肺炎支原体抗性的研究,选取17只2月龄健康的中国美利奴羊,14只为试验组,3只为对照组。相同饲养条件下人工感染绵羊肺炎支原体,于攻毒前1周、攻毒后3周观察临床症状,并通过ELISA检测羔羊血清MBL水平,结果显示,试验组攻毒后不同个体MBL含量水平整体下降。宰杀后进行组织病理学评分,重度、中度、轻度病理变化的评分分别为18分以上、12分左右、9分左右,该评分结果与临床观察结果一致,可知攻毒前血清MBL水平越高评分越低。表明MBL对绵羊肺炎支原体有抗性。
译  名:
Change of MBL in serum and histopathologic analysis after artificially infecting Mycoplasma ovipneumoniae
作  者:
WANG Xiao-yi;ZHANG Hong-mei;ABUDUREMU·Gu-lihan;GUO Cui-cui;ZHAO Zong-sheng;College of Life Science,Shihezi University;The First Affiliated Hospi-tal of Medicine College;College of Animal Science&Technology,Shihezi University;NO.1 senior middle school of qingzhou;
关键词:
sheep;;mannose-binding lectin;;histopathologic scores;;Mycoplasma ovipneumoniae
摘  要:
The aim of this study was to analyze mannose-binding lectin resistance to Mycoplasma ovipneumoniae,we selected17 healthy Chinese Merino sheep as subjects,3 of which were the control group.The 14 sheep as experimental group were artificially infected Mycoplasma ovipneumoniae,the levels of MBL in serum were quantitatively assayed by ELISA in 1 week before infection and 3 week after infection,respectively. The results showed that the MBL content of the experimental group dropped down as a whole and lambs decreased resistance to infection. Histopathologic scores were oabtained after sacrificing lambs,the scores of severe,moderate,mild pathological changes separately were 18 points or more,about 12 points,about 8 points,which were consistant with clinical observations,then we could know that the higher the levels of MBL in serum,the lower the scores. The results above suggested MBL pathway played a certain role in sheep Mpcoplasma pneumonia.

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