当前位置: 首页 > 文章 > 浦东白猪毛色的遗传基础研究——KIT基因变异的检测 上海交通大学学报(农业科学版) 2008,26 (2) 101-103+118
Position: Home > Articles > Genetic Basis of Hair Color of Pudong White Pig:Detecting Genetic Variation of KIT Gene Journal of Shanghai Jiaotong University(Agricultural Science) 2008,26 (2) 101-103+118

浦东白猪毛色的遗传基础研究——KIT基因变异的检测

作  者:
盛中华;白春艳;王起山;俞沛初;潘玉春;储野根;马永明;沈大德
单  位:
上海交通大学农业与生物学院;上海市南汇区种畜场
关键词:
浦东白猪;KIT基因;白毛色;PCR—SSCP
摘  要:
浦东白猪是国内唯一一个毛色全白的地方猪种。但是对其毛色全白的分子遗传基础,迄今一直无人研究。为此,本研究通过聚合酶链式反应-单链构象多态性分析(PCR-SSCP)以及对KIT基因内含子17和内含子18的测序探讨了浦东白猪白毛色的遗传控制体系与长白猪和大白猪白毛色控制体系的异同。结果证明,浦东白猪与长白猪和大白猪一样,其白毛色均为KIT基因内含子17发生了G→A的突变,内含子18的序列中存在AGTT的缺失造成的。
译  名:
Genetic Basis of Hair Color of Pudong White Pig:Detecting Genetic Variation of KIT Gene
作  者:
SHENG Zhong-hua~1 BAI Chun-yan~1 WANG Qi-shan~1 YU Pei-chu~1 PAN Yu-chun~1 CHU Ye-gen~2 MA Yong-ming~2 SHEN Da-de~2 (1.School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China;2.Shanghai Nanhui Pig Breeding Farm,Shanghai 201318,China)
关键词:
Pudong White pig;;KIT gene;;white hair color;;PCR-SSCP
摘  要:
Pudong White pig is the only one Chinese indigenous pig breed with full white hair color.However,the molecular genetic basis of this trait is unclear until now.In this study,we used the PCR-SSCP and DNA sequencing techniques to detect the variation of intrun17 and intrun18 of KIT gene which was already proved to be an important gent in controlling the hair color of Landrace and Large White.The results demonstrate that Pudong White also has the G→A mutation in intrunl7 and the AGTT deletion in intron 18 of KIT gene which is totally same with what in Landrace and Large White.

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