作 者：
唐丹;苏胜彦;朱文彬;王兰梅;宋飞彪;董娟娟;张丹;董在杰
单 位：
中国水产科学研究院淡水渔业研究中心农业农村部淡水渔业与种质资源利用重点实验室;南京农业大学无锡渔业学院
关键词：
红罗非鱼;竹炭;肌肉;脂肪酸组分;脂代谢基因
摘 要：
为研究饲料中添加竹炭对红罗非鱼幼鱼肌肉营养成分及脂代谢相关基因表达量的影响,实验选取平均体质量为(7.07±0.01) g的健康红罗非鱼幼鱼,随机分为5组,进行网箱养殖实验,每组3个重复,饲养60 d。饲料中竹炭添加量依次为0%、0.5%、1%、2%、4%。结果显示,0.5%、1%竹炭组的血清谷丙转氨酶(ALT)活性比对照组显著降低;碱性磷酸酶(ALP)和血清总蛋白(TP)活性在1%组显著升高;谷草转氨酶(AST)、甘油三酯(TG)和血清总胆固醇(TC)实验组比对照组显著降低;高密度脂蛋白(HDL)随着竹炭浓度的增加显著升高。1%竹炭组肌肉水分含量显著低于对照组;肌肉粗蛋白含量1%、2%和4%处理组显著低于对照组;与对照组相比,1%与4%的组中粗脂肪和肌肉灰分含量显著增加。饲料添加竹炭能提高鱼体肌肉中总不饱和脂肪酸(UFA)含量,但只有4%组的总不饱和脂脂蛋白脂肪酶(LPL)基因和苹果酸脱氢酶(MDH)基因表达量与对照组相比均有所升高,但是只有1%组的MDH基因与对照组相比有显著性差异。研究表明,饲料添加适当浓度的竹炭能提高鱼肉中粗脂肪、总不饱和脂肪酸(UFA)含量,以及LPL基因和MDH基因的表达,这将为提高红罗非鱼肉质提供新的途径。
译 名：
Effects of dietary addition of bamboo charcoal on muscle fatty acid composition and lipid metabolism related genes of juvenile red tilapia(Oreochromis mossambicus × O. niloticus)
作 者：
TANG Dan;SU Shengyan;ZHU Wenbin;WANG Lanmei;SONG Feibiao;DONG Juanjuan;ZHANG Dan;DONG Zaijie;Wuxi Fisheries College, Nanjing Agricultural University;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Freshwater Fisheries Research Centre, Chinese Academy of Fishery Sciences,Ministry of Agriculture and Rural Affairs;
关键词：
Oreochromis mossambicus × O.niloticus;;bamboo charcoal;;muscle;;fatty acid composition;;lipid metabolism gene
摘 要：
In order to investigate the effects of feed bamboo charcoal on muscle nutrition composition and lipid metabolism-related gene expression in juvenile red tilapia, the healthy juvenile red tilapia with an average body weight of(7.07±0.01) g were randomly divided into 5 groups, with 3 replicates in each group and were kept for 60 days. The quantity of bamboo charcoal in the feed was 0%, 0.5%, 1%, 2% and 4%, respectively. The results showed that: the activity of ALT in the 0.5% and 1% groups was significantly lower than that in the control group.The activity of alkaline phosphatase(ALP) and serum total protein(TP) activities were significant increased in the1% group. The aspartate aminotransferase(AST), triglyceride(TG) and serum total cholesterol(TC)concentrations in the experimental groups were significantly lower than those in the control group. High density lipoprotein(HDL) increased significantly with the increase of bamboo charcoal concentration. 1% bamboo charcoal group muscle moisture content was significantly lower than the control group; crude protein content of1%, 2% and 4% treatment groups was significantly lower than that of the control group. Crude fat content and muscle ash that of increased compared with the control group, 1% and 4% groups significantly increased compared with the control group. Feed bamboo charcoal can increase the content of total unsaturated fatty acid(UFA) in fish muscle, but only 4% group's total unsaturated fatty acid content increased significantly, on the contrary, the content of saturated fatty acids(SFA) in 4% group was significantly lower than that of the control group; C18: 2 and C18:3 n3 treatment in 4% bamboo charcoal treatment group increased significantly compared with the control group,and the C22: 6 in 1% bamboo charcoal treatment group was significantly lower than the control group. With the increase of bamboo charcoal feed, LPL gene and MDH gene expression increased compared with the control group, but only 1% group MDH gene compared with the control group were significantly different. Studies have shown that adding appropriate concentrations of bamboo charcoal can improve the crude fat content, total unsaturated fatty acid(UFA) content, LPL gene and MDH gene expression in fish, and help to change the nutrient composition of fish.
